ArticleMolecular biology of the cell2025
Functional fluorescence labeling of actins in live-cell system using engineered split-GFP technology.
Article in Molecular biology of the cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Actin filaments play essential roles in various cellular processes, and understanding their dynamics is crucial for studying cellular behaviors and actin-related diseases. However, conventional methods for visualizing actins often perturb its functionality or lack sufficient resolution for real-time imaging. In this study, we developed a method for functional fluorescence labeling of actin isoforms using split-GFP (Green fluorescent protein) technology, specifically through insertion of a GFP11 tag into a flexible residue pair (T229/A230) of human β-actin (ACTB) or γ-actin (ACTG). This strategy (GFP11-i) was successfully applied to visualize actin dynamics in mammalian cell lines, including the effects of disease-related mutations (e.g., ACTB R196H and ACTG S155F). In addition, we demonstrated the labeling of actin filaments in
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