Functional MRI brain state occupancy in the presence of cerebral small vessel disease

We aimed to replicate recent findings on the association between the extent of cerebral small vessel disease (cSVD), functional brain network dedifferentiation, and cognitive impairment. We analyzed demographic, imaging, and behavioral data from the prospective population-based Hamburg City Health Study. Using a fully prespecified analysis pipeline, we estimated discrete brain states from structural and resting-state functional magnetic resonance imaging (MRI). In a multiverse analysis, we varied brain parcellations and functional MRI confound regression strategies. The severity of cSVD was operationalized as the volume of white matter hyperintensities of presumed vascular origin. Processing speed and executive dysfunction were quantified using the Trail Making Test (TMT). We hypothesized a) that a greater volume of supratentorial white matter hyperintensities would be associated with less time spent in functional MRI-derived brain states of high fractional occupancy; and b) that less time spent in these high-occupancy brain states associated with a longer time to completion in part B of the TMT. High-occupancy brain states were characterized by activation or suppression of the default mode network. Every