ReviewJournal of biological methods2025
Pre-analytical considerations for microRNA quantification in childhood leukemia research.
Review in Journal of biological methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- MicroRNA-Mediated Regulation of Brain Aging Hallmarks: Implications for Neurodegeneration and Neural Recovery.Brain and behavior · 2026Review
- Recent Advances in Thalassemia Research: A Comprehensive Assessment From Diagnostic Technologies to Clinical Treatment.Journal of clinical laboratory analysis · 2026Review
- AutoML identification of microRNA biomarkers in high-risk pediatric acute lymphoblastic leukemia.Non-coding RNA research · 2025Article
- Unveiling the role of microRNAs, the PI3K/AKT pathway, and their associations in various types of leukemia: a comprehensive review.Blood research · 2025Review
- Association ofCurrent issues in molecular biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: MicroRNAs (miRNAs) have gained significant attention as potential biomarkers in childhood leukemia, offering insights into diagnosis, prognosis, and therapeutic targeting. However, the clinical translation of miRNA biomarkers faces several challenges, including inconsistencies in results caused by methodological differences, sample processing variability, and a lack of standardized normalization techniques. In addition, miRNA profiles are highly cell-specific, with unique signatures for different blood cell types and leukemic blasts, reflecting their distinct biological roles and disease states. Pre-analytical factors are critical in ensuring the accuracy and reproducibility of miRNA quantification. The selectively enriched and highly stable exosomal miRNAs have shown great promise for studying intercellular communication and disease-specific miRNAs. The selection of the analytical matrix should align with the specific objectives of the research or diagnostic application. Addressing technical challenges and recording potential confounding variables ( Objective: This review aims to highlight the challenges facing miRNA quantification in childhood leukemias, scrutinizing all relevant pre-analytical issues, including the preferred specimen source, leukemic blast type and count, the selection of suitable blood components, the impact of time and freeze-thaw cycles, collection, processing, and storage variables. Conclusion: Further research is needed to standardize methodologies and expand our understanding of miRNA interaction networks, ultimately advancing the application of miRNAs in childhood leukemia research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.