Evidence map›Paper›PMID 40799544›Full record

ArticlebioRxiv : the preprint server for biology2025

Miniaturized widefield microscope for high speed in vivo voltage imaging.

Catherine A Saladrigas, Forest Speed, Alec Teel, Mo Zohrabi, Eduardo J Miscles, Gregory L Futia, Larry V Baker, Ye Zhang, Ioannis Kymissis, Victor M Bright and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Catherine A SaladrigasDepartment of Electrical, Energy and Computer Engineering, University of Colorado Boulder, CO 80309,USA.ORCID 0000-0001-5060-1996
Forest SpeedDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Alec TeelDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Mo ZohrabiDepartment of Electrical, Energy and Computer Engineering, University of Colorado Boulder, CO 80309,USA.ORCID 0000-0003-2158-5661
Eduardo J MisclesDepartment of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USA.ORCID 0000-0002-1990-5032
Gregory L FutiaDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Larry V BakerDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Ye ZhangDepartment of Electrical Engineering, Columbia University, New York, NY 10027, USA.
Ioannis KymissisDepartment of Electrical Engineering, Columbia University, New York, NY 10027, USA.ORCID 0000-0001-7417-1759
Victor M BrightDepartment of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USA.ORCID 0000-0001-5498-3071
Cristin G WelleDepartment of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, CO 80045,USA.ORCID 0000-0001-8735-0983
Diego RestrepoDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.ORCID 0000-0002-4972-446X
Juliet T GopinathDepartment of Electrical, Energy and Computer Engineering, University of Colorado Boulder, CO 80309,USA.ORCID 0000-0003-2387-5371
Emily A GibsonDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.ORCID 0000-0002-3436-1483

Funding

Development of 3D-FAST Optical Interface for Rapid Volumetric Neural Sensing and ModulationR01NS123665 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GIBSON, EMILY, WELLE, CRISTIN G · 2021 to 2021
$2.7M
NINDS NIH HHS R01 NS123665
6 · The paper itself

Abstract

Functional imaging in freely moving animals with genetically encoded voltage indicators (GEVIs) will open new capabilities for neuroscientists to study the behavioral relevance of neural activity with high spatial and temporal precision. However, miniaturization of an imaging system with sufficient collection efficiency to resolve the small changes in fluorescence yield from voltage spikes, as well as development of efficient image sensors that are sufficiently fast to capture them, has proven challenging. We present a miniaturized microscope designed for voltage imaging, with a numerical aperture of 0.6, 250 μm field of view and 1.3 mm working distance that weighs 16.4 g. We show it is capable of imaging in vivo voltage spikes from Voltron2 with a spike peak-to-noise ratio >3 at a framerate of 530 Hz.

Identifiers

PMID40799544
PMCPMC12340831

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.