Evidence map›Paper›PMID 40798094›Full record

ArticleOptics express2025

MetaMax: an objective-sized instrument for advanced light microscope characterization and performance metadata collection.

Selene Flemming, David Grunwald

Abstract read
In one paragraph

Article in Optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Selene Flemming
David Grunwald

Funding

Visualizing local and global chromatin architecture, and gene expression in the individual cell by structural (SUSHI) and temporal (3D-SMRT) single molecule imagingU01DA047733 · NIDA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GRUNWALD, DAVID · 2018 to 2019
$1.0M
NIDA NIH HHS U01 DA047733
6 · The paper itself

Abstract

MetaMax is a device designed to replace the objective lens in light microscopes and provide a wide range of performance metadata that characterize microscope hardware behavior. MetaMax includes a large-area photodiode that is referenced against calibrated power meters to measure excitation light power and source stability; a broadband LED to quantify detector system responsivity and signal-dependent noise; a spectrometer to identify excitation wavelengths; an adjustable iris to simulate an objective's back aperture; and a quadrant photodiode to assess beam alignment and aperture overfill. MetaMax enables users to collect performance metadata for quality control, image provenance, and comprehensive acquisition parameter delineation. It simplifies access to performance data that typically requires expert knowledge and expensive equipment to obtain, lowering the access barrier for users. Such performance data is crucial metadata for improving the reliability and reproducibility of microscopy images. The MetaMax aims to facilitate collaboration through data reusability and promote a more rigorous, transparent approach to scientific inquiry using light microscopy techniques. This work presents a detailed evaluation and comparison of several MetaMax prototypes.

Identifiers

PMID40798094
PMCPMC12011381

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.