Evidence map›Paper›PMID 40797270›Full record

ArticleACS chemical biology2025

A Simple, Quick, and Scalable Route to Fluorogenic Ubiquitin and Ubiquitin-Like Protein Substrates for Assessing Activities of Deubiquitinases and Ubiquitin-Like Protein-Specific Proteases.

Saibal Chanda, Alan Pham, Yifan Shi, Sandeep Atla, Wenshe Ray Liu

Abstract read
In one paragraph

Article in ACS chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Synthetic Strategies for Activity-Based Probes to Decode Ubiquitin-Like Modifiers.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Saibal ChandaDepartment of Biochemistry and Biophysics, College of Agriculture and Life Sciences, Texas A&M University, College Station, Texas 77843, United States.ORCID 0000-0001-5884-5477
Alan PhamTexas A&M Drug Discovery Center and Department of Chemistry, College of Arts and Sciences, Texas A&M University, College Station, Texas 77843, United States.ORCID 0009-0002-4066-0110
Yifan ShiTexas A&M Drug Discovery Center and Department of Chemistry, College of Arts and Sciences, Texas A&M University, College Station, Texas 77843, United States.ORCID 0000-0002-8155-1351
Sandeep AtlaTexas A&M Drug Discovery Center and Department of Chemistry, College of Arts and Sciences, Texas A&M University, College Station, Texas 77843, United States.
Wenshe Ray LiuDepartment of Biochemistry and Biophysics, College of Agriculture and Life Sciences, Texas A&M University, College Station, Texas 77843, United States.ORCID 0000-0002-7078-6534

Funding

Use of the Noncanonical Amino Acid Mutagenesis Technique in Combination with Other Approaches to Study Functions of Posttranslational Lysine Modifications in ProteinsR35GM145351 · NIGMS · TEXAS A&M UNIVERSITY · PI Wenshe Ray Liu · 2022 to 2026
$2.6M
NIGMS NIH HHS R35 GM145351
6 · The paper itself

Abstract

Ubiquitin (Ub) and ubiquitin-like proteins (UBLs) regulate essential cellular processes as protein modifiers. While Ub signaling is well studied, many UBL pathways remain poorly defined, partly due to the limited availability of suitable UBL substrates. Here, we report the synthesis of fluorogenic Ub-ACA and UBL-ACA probes using the activated cysteine-based protein ligation (ACPL) technique to conjugate recombinant Ub and UBLs containing a

Indexed as

Deubiquitinating EnzymesFluorescent DyesUbiquitinUbiquitinsUbiquitin-Specific ProteasesCysteineHumansSubstrate SpecificityCysteineDeubiquitinating EnzymesFluorescent DyesUbiquitinUbiquitinsUbiquitin-Specific Proteases

Identifiers

PMID40797270
PMCPMC12455564

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.