Evidence map›Paper›PMID 40796885›Full record

ArticleJournal of nanobiotechnology2025

3D cell-laden scaffold printed with brain acellular matrix bioink.

Aobo Zhang, Siyu Zhu, Boyu Sun, Chengrui Nan, Lulu Cong, Zongmao Zhao, Liqiang Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Aobo ZhangDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Siyu ZhuDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Boyu SunDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Chengrui NanDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Lulu CongDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Zongmao ZhaoDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.
Liqiang LiuDepartment of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China. 27400950@hebmu.edu.cn.

Funding

Central Guiding Local Science and Technology Development Fund Projects 236Z7752GSpecial Project for the Construction of Hebei Province International Science and Technology Cooperation Base 193977143Dthe Medical Research Project of Hebei Provincial Health Commission 20230031
6 · The paper itself

Abstract

backgroundIntracerebral hemorrhage (ICH) is a severe neurological disorder characterized by bleeding within the brain tissue, typically associated with factors such as hypertension, cerebrovascular disease, and trauma. The transplantation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) has demonstrated promising effects in restoring neurological function in ICH rats; however, limited retention of these cells significantly impedes their efficacy. To address this limitation, we developed a bioink composed of decellularized extracellular matrix (dECM) and hUCMSCs, which was synthesized into 3D cell-laden scaffold through 3D bioprinting. This approach aims to extend the retention of hUCMSCs and create an early vascular microenvironment, thereby partially compensating for the drawbacks of hUCMSC transplantation and improving neurological function in ICH rats.

methodsThis study aimed to explore the use of a bioink formed by mixing 15% gelatin and 3% sodium alginate with a dECM solution, in conjunction with hUCMSCs, for 3D bioprinting of 3D cell-laden scaffold. The viscosity, morphology, and biocompatibility of the bioink were characterized using rheological analysis, scanning electron microscopy (SEM), and hematoxylin and eosin (HE) staining. Following printing, a live/dead assay kit was employed to assess the viability of hUCMSCs within the 3D cell-laden scaffold. ICH model rats were randomly assigned to four groups: (1) SHAM group; (2) ICH group; (3) ICH + 3D biological scaffold group; and (4) ICH + 3D cell-laden scaffold group.

resultshUCMSCs exhibited a higher retention rate within the 3D bioprinted 3D cell-laden scaffold. HE staining, immunohistochemistry, and immunofluorescence results indicated that the 3D biological scaffold encapsulating hUCMSCs had a significant impact on the vascularization of the printed 3D cell-laden scaffold. Furthermore, 3D cell-laden scaffold improved nerve function and promoted angiogenesis in rats with cerebral hemorrhage better than 3D biological scaffolds.

conclusionOur results suggest that 3D bioprinted 3D cell-laden scaffold hold great potential for restoring impaired neurological function in ICH rats.

Indexed as

BioprintingBrainDecellularized Extracellular MatrixPrinting, Three-DimensionalTissue ScaffoldsAlginatesAnimalsCell SurvivalCerebral HemorrhageExtracellular MatrixHumansInkMaleMesenchymal Stem CellsRatsRats, Sprague-DawleyAlginatesDecellularized Extracellular Matrix3D bioprinting3D cell-laden scaffoldAngiogenesisDecellularized extracellular matrixHuman umbilical cord-derived mesenchymal stem cells

Identifiers

PMID40796885
PMCPMC12344831

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.