ArticleJournal of animal science2025
Salmate DHA+ fish oil supplement fed to boars and impact on sperm production.
Article in Journal of animal science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Omega-3 fatty acid supplementation can increase sperm production in boars, but the mechanism has not been demonstrated. The current study was to determine if the increase in sperm production was due to a reduction in germ cell apoptosis during spermatogenesis. Duroc boars (438-686 d of age) were fed either a control diet of corn, soybean meal, 5% fiber, plus vitamins and minerals per NRC requirements or the control diet plus a proprietary blend of fish oil, Salmate DHA+ supplemented diet with 1.83 g DHA (Docosahexaenoic Acid, C22:6) and 0.78 g EPA (Eicosatetraenoic acid, c20:5) omega-3 fatty acids from 26 g Salmate DHA+ (The Ballard Group, Inc. and Feedworks USA, Ltd.) per day per head added to the control diet. The control diet was fed to all boars (n = 10) for 62 d, and then Salmate DHA+ was added to the diet of 5 of the boars while the other 5 continued the control diet for another 62 d. At the end of the experiment, all boars were harvested, the testes collected, fixed with Karnovsky's fixative, postfixed with 70% ethanol, embedded with acrylic resin, 2-micron sections taken, and sections stained with Toluidine Blue. During the study, boars were on routine semen collection. Semen data were evaluated for all boars on the first 62 d of the control diet (before period) and then for control and Salmate DHA+ boars from days 22 to 62 after Salmate DHA+ feeding started (after period). A total of 107 ejaculates were evaluated in the study. The control boars' total sperm per ejaculate (×109) was 105.7 ± 7.3 vs. 110.9 ± 10.4 in the before vs. after period (p > 0.05). The Salmate DHA+ boars' total sperm per ejaculate (×109) was 103.4 ± 5 vs. 122.8 ± 7 in the before vs. after period (1.18-fold increase, P < 0.05). To examine apoptosis via histology, the number of round spermatids (RS) and Sertoli cells (SC) were counted in 10 stage 1 seminiferous tubules for each boar with adjustments made for cell nucleoli or nuclei size and thickness of the histology section. The ratio of RS/SC or Sertoli Cell Index was then determined to estimate how many RS survived apoptosis in spermatogenesis. The Sertoli Cell index was 9.46 ± 0.84 vs. 15.03 ± 0.97, respectively, for Control and Salmate DHA+ boars (P < 0.01). The number of RS/SC increased 1.59-fold due to Salmate DHA+ treatment and demonstrates a reduction in apoptosis. The increased number of RS may explain the increased sperm per ejaculate after Salmate DHA+ treatment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.