Evidence map›Paper›PMID 40795157›Full record

ArticleThe Journal of infectious diseases2026

Emergence of ST3390: A Novel Apigmented MRSA Clone From the CC5 Lineage.

Emily A Felton, Mary-Elizabeth Jobson, Nathanial J Torres, Rachel M Washburn, Ariana M Virgillio, Joshua Alvior, Eleonora Cella, Amorce Lima, Deanna Becker, Suzane Silbert and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Emily A FeltonDepartment of Molecular Biosciences, University of South Florida, Tampa, Florida, USA.
Mary-Elizabeth JobsonDepartment of Molecular Biosciences, University of South Florida, Tampa, Florida, USA.
Nathanial J TorresDepartment of Molecular Biosciences, University of South Florida, Tampa, Florida, USA.
Rachel M WashburnDepartment of Molecular Biosciences, University of South Florida, Tampa, Florida, USA.ORCID 0009-0009-8357-9962
Ariana M VirgillioDivision of Infectious Disease and Internal Medicine, University of South Florida, Tampa, Florida, USA.ORCID 0009-0003-2431-6915
Joshua AlviorDivision of Infectious Disease and Internal Medicine, University of South Florida, Tampa, Florida, USA.
Eleonora CellaBurnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, USA.
Amorce LimaEsoteric Testing/R&D Laboratory, Tampa General Hospital, Tampa, Florida, USA.
Deanna BeckerEsoteric Testing/R&D Laboratory, Tampa General Hospital, Tampa, Florida, USA.
Suzane SilbertEsoteric Testing/R&D Laboratory, Tampa General Hospital, Tampa, Florida, USA.
Taj AzarianBurnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, USA.ORCID 0000-0002-8611-5241
Kami KimCenter for Antimicrobial Resistance, University of South Florida, Tampa, Florida, USA.ORCID 0000-0003-3384-152X
Lindsey N ShawDepartment of Molecular Biosciences, University of South Florida, Tampa, Florida, USA.ORCID 0000-0002-0629-8990

Funding

Dissecting the Influence of a C-Terminal Processing Protease on S. aureus PathogenesisR01AI157506 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI SHAW, LINDSEY NEIL · 2021 to 2024
$1.5M
National Institute of Allergy and Infectious DiseasesNIAID NIH HHS R01 AI157506USF Provost's CREATE
6 · The paper itself

Abstract

backgroundOne of the most successful and widely-distributed hospital-associated lineages of MRSA is clonal complex 5 (CC5). These strains are known for widespread antibiotic resistance but less severe disease than CA-MRSA counterparts. Recently, CC5 descendant lineages have appeared globally with hypervirulent properties. Herein, we identify and characterize a rare and novel CC5 MRSA sequence type, ST3390.

methodsWe used whole genome sequencing, alongside phenotypic characterizations, genetic complementation, blood viability- and neutrophil-killing assays, and a murine model of sepsis to study the pathogenic capabilities of ST3390 strains.

resultsTo date, there have only been 65 recorded instances of infection caused by ST3390 globally, with 36 of those occurring in Tampa (TPA-ST3390). Genomic analysis of strains identified numerous spa-types, with a t010 cluster found only in our strains. Exploration of AMR genes detected the presence of unique hybrid SCCmec types, with ∼90% of Tampa strains possessing components of SCCmecIa, SCCmecIIa, and/or SCCmecVIII. Phenotypically, all ST3390 strains lack the staphyloxanthin pigment, which is mediated by a conserved 6aa in frame deletion within the staphyloxanthin biosynthesis protein CrtN. TPA-ST3390 strains display high levels of cytotoxicity towards human neutrophils compared with other CC5 lineages and are also virulent in animal models of infection.

conclusionsThis is the first study to characterize the pathogenicity and genomic architecture of the rare MRSA lineage ST3390. Our work provides a deeper understanding of the clonal expansion of CC5, and the wider diversification of Staphylococcus aureus isolates within patient populations.

Indexed as

Methicillin-Resistant Staphylococcus aureusStaphylococcal InfectionsAnimalsAnti-Bacterial AgentsDisease Models, AnimalFemaleGenome, BacterialHumansMiceNeutrophilsSepsisVirulenceWhole Genome SequencingAnti-Bacterial AgentsMRSAmupirocinStaphylococcus aureus

Identifiers

PMID40795157
PMCPMC12811862

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.