ArticleMolecular carcinogenesis2025
Improved Methods for the Stable Generation of Human Papillomavirus-Driven Head and Neck Cancer Cell Lines.
Article in Molecular carcinogenesis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- The utility of fibroblast co-culture for the maintenance of episomes in human papillomavirus-associated cancer models.mSphere · 2026Article
- The Utility of Fibroblast Co-culture for the Maintenance of Episomes in Human Papillomavirus-Associated Cancer Models.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Cell lines are an essential tool in research, leading to new discoveries involving drug studies, prognosis, treatment outcomes, genomic abnormalities, and cellular pathway deviations. There is an ongoing need for new cell lines in cancer research. Cell lines are often initiated by using tissue explants or dissociating cells onto plastic; this proves ineffective with challenging cell lines. Here we report two improved protocols in establishing stable cell lines. The first takes the more classical approach but is paired with fluorescence-activated cell sorting (FACs). The second protocol involves coculturing with feeder cells, coupled with FACs. We demonstrate the use of these protocols in the establishment of 17 cell lines, including several cancer associated fibroblasts (CAFs) and a human papilloma virus (HPV)- positive HNSCC cell line.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.