ArticleInvestigative ophthalmology & visual science2025
DNA 5-Hydroxymethylcytosine Landscape and Transcriptional Profile Highlight the TUBB4B-Mediated Th17/Th1/Treg Imbalance in Behçet's Uveitis.
Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Astragaloside IV targets TUBB4B to inhibit proliferation and promote apoptosis of pituitary tumor cells via the STMN1/ERK pathway.International journal of molecular medicine · 2026Article
- Integrative single-cell transcriptomics and mendelian randomization identifies BTN3A2 as a shared protective factor in Behçet's disease and inflammatory bowel disease.Frontiers in immunology · 2026Article
- Regulatory T cell dysfunction and exhaustion in uveitis: immunometabolic mechanisms, microenvironmental drivers, and emerging therapeutic strategies.Frontiers in immunology · 2026Review
- Causal effects of antibody-mediated immunity to pathogens on five ophthalmic diseases: a Mendelian randomization study.Experimental biology and medicine (Maywood, N.J.) · 2026Article
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Authors and funding
13 authors.
Funding
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Abstract
Purpose: Behçet's uveitis (BU) is an auto-inflammatory disease frequently with a poor prognosis. Here, we performed an integrated analysis of DNA 5-hydroxymethylcytosine (5-hmC) landscape and transcriptomic profiling in CD4+ T cells from active BU patients and healthy controls. Methods: We conducted an integrated analysis of DNA 5-hmC modifications and transcriptomic data from CD4+ T cells of active BU patients and healthy individuals. Publicly available single-cell RNA-sequencing data were analyzed to validate findings across cell clusters. Functional experiments were performed to assess the effects of TUBB4B on cytokine production and T-cell frequencies. Results: Bioinformatics analyses identify 801 downregulated and 2489 upregulated differential hydroxymethylated genes, predominantly enriched in pathways related to pathogen defense and various immune responses. RNA sequencing reveals 958 downregulated and 572 upregulated genes, with significant enrichment in pathways related to bacterial infection. Integration of epigenetic and transcriptional data highlights 74 candidate genes and three genes including TUBB4B, SKI and ZFPM1 are validated to be downregulated in active BU patients. Further analysis of publicly available single-cell RNA-sequencing data reveals TUBB4B downregulation across multiple cell clusters, particularly in naïve CD4+ T cells. Functional experiments indicate that overexpressing TUBB4B could decrease the frequencies of IL-17+ and IFN-γ+ CD4+ T cells and reduce the production of IL-17 and IFN-γ, while upregulating the frequency of CD4+CD25+FOXP3+ T cells and enhancing secretion of IL-10. Conclusions: This study underscores the pivotal role of pathogen-induced immune dysregulation in BU development and identifies TUBB4B as a potential therapeutic target for the study on prevention and treatment of this disease.
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