Evidence map›Paper›PMID 40792510›Full record

ArticleMicrobiology spectrum2025

Nested PCR to optimize

Laëtitia Leclerc, Gabryelle Agoutin, Thierry Brévault, Antony Champion, Jean Mainguy, Nicolas Nègre, Sudeeptha Yainna, Géraldine Pascal, Sophie Gaudriault, Jean-Claude Ogier

Abstract read
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Laëtitia LeclercDGIMI, INRAE, Univ Montpellier, Montpellier, France.
Gabryelle AgoutinGenPhySE, INRAE, ENVT, Université de Toulouse, Castanet Tolosan, France.
Thierry BrévaultAIDA, CIRAD, Univ Montpellier, Montpellier, France.
Antony ChampionDIADE, IRD, Univ Montpellier, Montpellier, France.
Jean MainguyINRAE, BioinfOmics, GenoToul Bioinformatics facility, Université de Toulouse, Castanet-Tolosan, France.
Nicolas NègreDGIMI, INRAE, Univ Montpellier, Montpellier, France.
Sudeeptha YainnaDGIMI, INRAE, Univ Montpellier, Montpellier, France.
Géraldine PascalGenPhySE, INRAE, ENVT, Université de Toulouse, Castanet Tolosan, France.
Sophie GaudriaultDGIMI, INRAE, Univ Montpellier, Montpellier, France.ORCID 0000-0003-2789-4959
Jean-Claude OgierDGIMI, INRAE, Univ Montpellier, Montpellier, France.ORCID 0000-0002-5439-694X

Funding

INRAE (France) MicroSpodoRiceThe Region Occitanie and the European Union FEDER-FSE 2014-2020
6 · The paper itself

Abstract

Housekeeping genes have proven to be effective taxonomic markers for characterizing bacterial microbiota in short-read amplicon metabarcoding studies. A region of the

Indexed as

BacteriaBacterial ProteinsDNA, BacterialDNA Barcoding, TaxonomicDNA-Directed RNA PolymerasesPolymerase Chain ReactionAnimalsDNA PrimersMicrobiotaRNA, Ribosomal, 16SBacterial ProteinsDNA, BacterialDNA-Directed RNA PolymerasesDNA PrimersRNA, Ribosomal, 16Shost-associated microbiotainsectmetabarcodingnested PCRrpoBSpodoptera frugiperda

Identifiers

PMID40792510
PMCPMC12403564

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.