Evidence map›Paper›PMID 40792501›Full record

ArticleMicrobiology spectrum2025

Single-cell analysis of yeast surface display for designer cellulosome applications using a fluorescent protein complex.

Babette Lamote, Emma Cremelie, Kristel Demeyere, Philippe De Groote, Dennis Grimon, Julie Vanderstraeten, Evelyne Meyer, Marjan De Mey, Yves Briers

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Discovery and functional characterization of endoglucanases fromApplied and environmental microbiology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Babette LamoteLaboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, Belgium.ORCID 0000-0002-0323-959X
Emma CremelieLaboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, Belgium.ORCID 0000-0003-0957-1443
Kristel DemeyereLaboratory of Biochemistry, Department of Veterinary and Biosciences, Ghent University, Merelbeke, Belgium.ORCID 0000-0002-3966-6825
Philippe De GrooteLaboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, Belgium.ORCID 0000-0002-1713-7981
Dennis GrimonLaboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, Belgium.ORCID 0000-0001-7298-6831
Julie VanderstraetenLaboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, Belgium.ORCID 0000-0002-3468-0927
Evelyne MeyerLaboratory of Biochemistry, Department of Veterinary and Biosciences, Ghent University, Merelbeke, Belgium.ORCID 0000-0002-7066-8255
Marjan De MeyCentre for Synthetic Biology, Department of Biotechnology, Ghent University, Ghent, Belgium.ORCID 0000-0002-8134-9436
Yves BriersLaboratory of Applied Biotechnology, Department of Biotechnology, Ghent University, Ghent, Belgium.ORCID 0000-0001-7723-1040

Funding

Bijzonder Onderzoeksfonds UGent BOF16/STA/024Bijzonder Onderzoeksfonds UGent BOF17/DOC/086Fonds Wetenschappelijk Onderzoek 1S15424NFonds Wetenschappelijk Onderzoek 1SE2623N
6 · The paper itself

Abstract

Designer cellulosomes (DCs) are precisely engineered multi-enzyme complexes aimed at lignocellulose saccharification. Achieving spontaneous designer cellulosome display on yeast cell surfaces has been a long-term objective to enhance consolidated bioprocesses with concurrent ethanol production. A "self-assembly" approach involves simultaneous scaffoldin display and docking enzyme secretion. However, challenges arise from the size and complexity of designer cellulosomes, coupled with the yeast cells' limited capacity for heterologous protein expression. A comprehensive examination of

Indexed as

CellulosomesLuminescent ProteinsSaccharomyces cerevisiaeSingle-Cell AnalysisEthanolFermentationLigninSaccharomyces cerevisiae ProteinsEthanolLigninlignocelluloseLuminescent ProteinsSaccharomyces cerevisiae Proteinsconfocal microscopydesigner cellulosomesflow cytometryfluorescent proteinsSaccharomyces cerevisiaeyeast surface display

Identifiers

PMID40792501
PMCPMC12403707

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.