ArticleAging pathobiology and therapeutics2024
Technical development of senescence-associated β-galactosidase staining in frozen kidney tissue of Sprague Dawley rat.
Article in Aging pathobiology and therapeutics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
To establish the optimal conditions for SA-β-galactosidase staining on frozen rat kidney tissue sections, we evaluated staining intensity, specificity, and consistency under several experimental conditions. We tested the effects of tissue freezing methods, fixative solutions and methods, section thickness, and duration of incubation time for SA-β-gal staining to obtain consistent results. This work was prompted by the emerging developments that strongly suggest the potential central roles of cellular senescence in aging and age-related diseases. To further examine the direct roles of senescent cell accumulation on age-related changes in various tissues and organs, it is essential to determine tissue localization and distribution, cell-type specificity, and direct correlations to histopathological changes. Furthermore, the recent advancements in molecular analyses, including spatial transcriptomics and MALDI-MSI spatial metabolomics,
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.