Evidence map›Paper›PMID 40790308›Full record

ArticleScientific reports2025

Comparative effects of standardized Centella asiatica extract (ECa 233) and its active compound mixture on proteomics and mitochondrial function.

Yingrak Boondam, Narawut Pakaprot, Meng Chieh Yang, Nichawadee Sandech, Arnatchai Maiuthed, Jutima Samer, Kanjanawadee Prasittisa, Jutamas Ruanpang, Chit Care, Srirat Chuayboon

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yingrak BoondamDepartment of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Narawut PakaprotDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand. narawut.pak@mahidol.ac.th.
Meng Chieh YangCentre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Nichawadee SandechCentre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Arnatchai MaiuthedCentre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Jutima SamerDepartment of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Kanjanawadee PrasittisaFaculty of Education, Nakhon Phanom University, Nakhon Phanom, Thailand.
Jutamas RuanpangDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Chit CareDepartment of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Srirat ChuayboonDepartment of Engineering, King Mongkut's Institute of Technology Ladkrabang, Prince of Chumphon Campus, Chumphon, Thailand.

Funding

Mahidol University A27/2563
6 · The paper itself

Abstract

ECa 233 is a standardized extract of Centella asiatica that contains the major active compounds madecassoside (MDS) and asiaticoside (ASS), which can strengthen hippocampal synapses, resulting in a memory-enhancing effect. This study explored the possible mechanisms of ECa 233 related to synaptic enhancement via proteomic analysis. Differentiated SH-SY5Y cells and hippocampal slices obtained from healthy male Wistar rats were treated with dimethyl sulfoxide, ECa 233, or the MDS + ASS mixture. Mitochondrial function and protein expression were examined via proteomic, western blot, and immunofluorescence analyses. Effects on synaptic plasticity were confirmed via electrophysiology using acute hippocampal slices. The treated groups exhibited a significant increase in mitochondrial activity and synaptic plasticity-related protein expression. Upstream Akt-mTOR signaling was significantly enhanced, with the most pronounced effects observed in the MDS + ASS group. However, the ECa 233 group still showed the highest hippocampal synaptic response. Based on our data, both ECa 233 and MDS + ASS were involved in mitochondrial metabolism, with the Akt-mTOR pathway as one of the targets promoting these activities. Furthermore, the synergistic effect of MDS + ASS was essential to achieve the same level of response obtained with ECa 233. Ultimately, the MDS + ASS mixture may enhance cellular plasticity, while the remaining components of ECa 233 may promote specific proteins that play a role in maintaining biological homeostasis.

Indexed as

MitochondriaPlant ExtractsTriterpenesAnimalsCell Line, TumorCentellaHippocampusHumansMaleNeuronal PlasticityProteomicsProto-Oncogene Proteins c-aktRatsRats, WistarSignal TransductionTOR Serine-Threonine KinasesasiaticosideCentella asiatica extractECa 233 extractmadecassosidePlant ExtractsProto-Oncogene Proteins c-aktTOR Serine-Threonine KinasesTriterpenesAsiaticosideMadecassosideMitochondriaProteomicSH-SY5YTriterpenoid glycosides

Identifiers

PMID40790308
PMCPMC12339980

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