Evidence map›Paper›PMID 40790101›Full record

ArticleEMBO molecular medicine2025

Protein-based tools for the detection and characterisation of Oropouche virus infection.

Monique K Merchant, Juliano de Paula Souza, Sahar Abdelkarim, Shrestha Chakraborty, Tufi A Nasser Neto, Kristel I Gutierrez Manchay, Daniel M de Melo Jorge, Valdinete Alves do Nascimento, Yuxian Sun, Eve R Caroe and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO molecular medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Monique K MerchantDepartment of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK.ORCID http://orcid.org/0000-0002-6783-7193
Juliano de Paula SouzaDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Sahar AbdelkarimThe Rosalind Franklin Institute, Harwell Science Campus, Didcot, OX11 0QX, UK.
Shrestha ChakrabortyDepartment of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK.ORCID http://orcid.org/0000-0001-9658-6701
Tufi A Nasser NetoDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.ORCID http://orcid.org/0009-0007-0532-5911
Kristel I Gutierrez ManchayDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Daniel M de Melo JorgeDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Valdinete Alves do NascimentoNúcleo de Vigilância de Vírus Emergentes, Reemergentes ou Negligenciados - ViVER/EDTA, Instituto Leônidas e Maria Deane, Fiocruz, Manaus, Amazonas, 69053-138, Brazil.
Yuxian SunDepartment of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK.ORCID http://orcid.org/0009-0002-2533-7745
Eve R CaroeDepartment of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK.
Lauren E A EyssenThe Rosalind Franklin Institute, Harwell Science Campus, Didcot, OX11 0QX, UK.ORCID http://orcid.org/0000-0003-4788-7203
Jared S RuddDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Isa C Ribeiro PiauilinoFundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Amazonas, 69040-000, Brazil.
Sérgio Damasceno PintoFundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Amazonas, 69040-000, Brazil.ORCID http://orcid.org/0000-0001-7034-4245
Matheus H Pereira da SilvaDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.ORCID http://orcid.org/0000-0002-9118-513X
Felipe Rocha do NascimentoDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Felipe Gomes NavecaNúcleo de Vigilância de Vírus Emergentes, Reemergentes ou Negligenciados - ViVER/EDTA, Instituto Leônidas e Maria Deane, Fiocruz, Manaus, Amazonas, 69053-138, Brazil.ORCID http://orcid.org/0000-0002-2888-1060
José L Proenca-ModenaLaboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, São Paulo, 13083-862, Brazil.ORCID http://orcid.org/0000-0002-4996-3153
Luis L P da SilvaDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Regina M Pinto de FigueiredoFundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, Amazonas, 69040-000, Brazil.
Raymond J OwensThe Rosalind Franklin Institute, Harwell Science Campus, Didcot, OX11 0QX, UK.
Eurico ArrudaDepartment of Cell Biology and Center for Virus Research, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, 14049-900, Brazil. eaneto@fmrp.usp.br.ORCID http://orcid.org/0000-0002-0978-410X
Stephen C GrahamDepartment of Pathology, University of Cambridge, Cambridge, CB2 1QP, UK. scg34@cam.ac.uk.ORCID http://orcid.org/0000-0003-4547-4034

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 19/26119-0Royal Society (The Royal Society) ICA\R1\201019UKRI | Biotechnology and Biological Sciences Research Council (BBSRC) BB_V018523
6 · The paper itself

Abstract

Oropouche fever is a neglected tropical disease caused by the orthobunyavirus Oropouche virus (OROV). A recent OROV epidemic caused by a novel reassortant has seen infections across an expanded geographical range, with deaths of healthy adults plus vertical transmission leading to pregnancy loss. OROV research and epidemiology is hampered by a paucity of available tools for serology and molecular virology. We have purified recombinant OROV nucleoprotein and the spike region of the viral surface glycoprotein Gc. These antigens detect seroconversion following experimental infection of animals in indirect ELISA, confirming their antigenic authenticity. They stimulate the production of high neutralising antibody titres in animals, highlighting their promise as immunogens for vaccination. We developed a nanobody-based sandwich ELISA that can detect OROV antigens in human clinical serum samples with high efficiency, and we show that nanobodies directed against OROV Gc spike can potently neutralise infection by both historical OROV strains and the newly emerged reassortant. Our protein-based reagents will accelerate OROV research and highlight the utility of protein-based tools for future OROV vaccines and point-of-care diagnostic devices.

Indexed as

Bunyaviridae InfectionsOrthobunyavirusAnimalsAntibodies, NeutralizingAntibodies, ViralAntigens, ViralEnzyme-Linked Immunosorbent AssayHumansRecombinant ProteinsSingle-Domain AntibodiesAntibodies, NeutralizingAntibodies, ViralAntigens, ViralRecombinant ProteinsSingle-Domain AntibodiesAmazonArbovirusBrazilNeglected Tropical Disease (NTD)Vector-borne Disease

Identifiers

PMID40790101
PMCPMC12423313

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.