Evidence map›Paper›PMID 40788455›Full record

ArticleProbiotics and antimicrobial proteins2026

Hematological, Histological, and NMR Analysis of Meat Quality and Growth Performance of Broilers Fed with Probiotics Bacillus licheniformis-Supplemented Poultry Feed.

Basit Ali Khan, Sundas Sharif, Amna Mehdi, Iram Liaqat, Sikander Ali, Asad-Ur-Rehman, Liangcai Peng, Sohail Afzal, Muhammad Nauman Aftab

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Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Basit Ali KhanDr. Ikram-Ul-Haq Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan.
Sundas SharifDr. Ikram-Ul-Haq Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan.
Amna MehdiDr. Ikram-Ul-Haq Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan.
Iram LiaqatDr. Nazir Ahmad Institute of Biological Sciences, Government College University Lahore, Lahore, Pakistan.
Sikander AliDr. Ikram-Ul-Haq Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan.
Asad-Ur-RehmanDr. Ikram-Ul-Haq Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan.
Liangcai PengKey Laboratory of Fermentation Engineering (Ministry of Education), Biomass & Bioenergy Research Center, Hubei University of Technology, Wuhan, 430068, China.
Sohail AfzalDepartment of Life Science, School of Sciences, University of Management and Technology, Lahore, 54770, Pakistan.
Muhammad Nauman AftabDr. Ikram-Ul-Haq Institute of Industrial Biotechnology, Government College University Lahore, Lahore, Pakistan. dr.naumanaftab@gcu.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study evaluated the effect of probiotics (Bacillus licheniformis) on the growth pattern, hematological parameters, metabolic profile, and meat quality of broiler birds. Total of 75 Ross 308 broiler chicks were divided into three groups (25 broilers in each) and fed with poultry feed supplemented with probiotics (15 g/ton of feed for starter diet and 35 g/ton for grower diet), antibiotic (amoxicillin 900 mg/ton of feed) and basal poultry feed, respectively, for 5 weeks. The broilers of control group exhibited higher feed conversion rate (FCR), i.e., 1.932 ± 0.10 followed by the FCR of antibiotic group (1.84 ± 0.10) and probiotic (1.714 ± 0.10) group broilers. It indicated that the chicks fed with probiotic rich feed gained more weight while ingesting comparatively less quantity of feed. The difference in weight of body organs (heart, liver, spleen, and gallbladder) among all groups showed no significant change (p > 0.05). However, significant difference of intestinal length was observed; control group with longest intestine (213.5 ± 15.22 cm) and probiotic group with shortest intestine of length 194 ± 5.51 cm. Moreover, probiotics group showed an improved temperature (30.75 ± 1.75 °C) and pH (6.05 ± 0.05) in meat after 20 min of slaughter. The breast muscles of probiotic group chickens exhibited greater cooking loss, yellowness (b*) (13.15 ± 2.23) and lightness (L*) (53.9 ± 1.6) with reduced redness (a*) (12.305 ± 1.35) and shear force (17.265 ± 0.82) compared to the control and antibiotic-treated group. The histological examination of probiotics group chickens pectoral muscles indicated significant (p < 0.05) increase in fascicle diameter (654.08 ± 5.55 µm) and fiber density (29.26 ± 0.65 n/µm) of meat compared to the antibiotics and control group. Probiotic-treated chickens showed improved intestinal health while the blood glucose level of the probiotics group chicken was reported to be higher. Metabolic analysis of leg and breast meat of chickens fed with probiotics supplemented feed with 600 MHz 1H-NMR spectra revealed 23 identified metabolites, of which seven metabolites (carnitine, phenylalanine, histidine, O-acetylcarnitine, omega 3 fatty acid, uridine, lactate) were only reported in probiotics group. The findings suggest that B. licheniformis enhances growth, carcass yield, organ weights, meat quality, and overall health of broiler birds. Consequently, Bacillus licheniformis can be utilized as a substitute growth promoter instead of antibiotics.

Indexed as

Animal FeedBacillus licheniformisChickensMeatProbioticsAnimal Nutritional Physiological PhenomenaAnimalsDietary SupplementsMaleBacillus licheniformisBroilerMeat qualityNMR analysisProbiotics

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.