Evidence map›Paper›PMID 40787988›Full record

ArticleJournal of virology2025

Expression of spike and hemagglutinin-esterase proteins is necessary to recover infectious recombinant bovine coronavirus.

Yoshiro Sugiura, Tatsuki Takahashi, Shiori Ueno, Sodbayasgalan Amarbayasgalan, Kenta Shimizu, Makoto Ujike, Tohru Suzuki, Wataru Kamitani

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yoshiro SugiuraDepartment of Infectious Disease and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan.
Tatsuki TakahashiDepartment of Infectious Disease and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan.
Shiori UenoDepartment of Infectious Disease and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan.
Sodbayasgalan AmarbayasgalanDepartment of Infectious Disease and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan.
Kenta ShimizuDepartment of Infectious Disease and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan.
Makoto UjikeLaboratory of Veterinary Infectious Diseases, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.ORCID 0000-0002-7145-7126
Tohru SuzukiDivision of Zoonosis Research, Sapporo Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido, Japan.
Wataru KamitaniDepartment of Infectious Disease and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan.ORCID 0000-0003-0165-9408

Funding

Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science 22K15467Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science 23K05542Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science 24K10242JST Moonshot Research and Development Program JPMJMS2025JST SPRING JPMJSP2146Regulatory Research Projects for Food Safety, Animal Health, and Plant Protection, Ministry of Agriculture, Forestry, and Fisheries of Japan JPJ008617. 21455165
6 · The paper itself

Abstract

Bovine coronavirus (BCoV) is a significant pathogen in cattle, and its virological analysis has been hampered by the difficulty in isolating and culturing field isolates with high titers. Here, we successfully generated recombinant BCoV using an infectious bacterial artificial chromosome DNA clone co-expressing the BCoV spike (S) and hemagglutinin-esterase (HE) proteins. We also investigated the role of trypsin in BCoV culture using a recombinant virus expressing the ZsGreen reporter gene (Rec-BCoV-Kakegawa-ZsGreen). We found that an optimized concentration of 2.5 µg/mL significantly enhanced viral titers, reaching 2 × 10

Indexed as

Coronavirus, BovineHemagglutinins, ViralSpike Glycoprotein, CoronavirusViral Fusion ProteinsAnimalsCattleChromosomes, Artificial, BacterialTrypsinVirus Replicationhemagglutinin esteraseHemagglutinins, ViralSpike Glycoprotein, CoronavirusTrypsinViral Fusion ProteinsBCoVreporter virusreverse genetics

Identifiers

PMID40787988
PMCPMC12456148

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.