ArticleAnalytical chemistry2025
Online Coupling of Acoustic Droplet Levitation with Capillary Electrophoresis Mass Spectrometry Enables Midair Enrichment for Improved Proteomic Sensitivity.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Aspiration Patch Proteomics Enables CE-ESI-MS Proteotyping of Identified Single Neurons in Intact Brain Tissue.Analytical chemistry · 2026Article
- Recent Developments and Applications of Capillary and Microchip Electrophoresis in Proteomics and Peptidomics (2023-2025).Journal of separation science · 2026Review
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Authors and funding
2 authors.
Funding
Abstract
Contact between analytes and surfaces during sample handling remains a major barrier to sensitivity in trace-level proteomics, including single-cell mass spectrometry (MS). Here, we introduce the first online integration of acoustic droplet levitation with capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESI-MS), enabling containerless, midair sample enrichment and analysis. In this Levitational CE-MS platform, droplets containing proteome digests were stably levitated and allowed to evaporate midair, without active acceleration, using an asymmetric acoustic levitator to concentrate analytes prior to CE-MS analysis. Optical imaging confirmed a ∼4.4-fold volume reduction after 60 min of passive evaporation, corresponding to ∼3.4-fold concentration enrichment based on label-free quantification of the resulting proteome. This enhancement increased sample utilization by ∼100% compared to conventional CE-ESI-MS, doubling protein identifications from ∼3 ng HeLa digest (∼10 cells) to 2169 proteins after enrichment (∼14 ng). Quantitation showed consistent proteome composition without concentration or hydrophobicity bias. These results establish Levitational CE-ESI-MS as a sensitive, reproducible, and contact-free strategy for trace-level proteomics, opening new possibilities for low-input including single-cell analyses.
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Registered trials
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