Evidence map›Paper›PMID 40787451›Full record

ArticleFrontiers in immunology2025

RAG recombinase expression discriminates the development of natural killer cells.

Jasmin Sprissler, Ulrich Pannicke, Eva-Maria Rump, Hubert Schrezenmeier, Nicolas Casadei, Michaela Pogoda, Laurence Kuhlburger, Morgana Barroso Oquendo, Stefan Czemmel, Klaus-Michael Debatin and 3 more

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Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jasmin SprisslerDepartment of Pediatrics and Adolescent Medicine, University Ulm Medical Center, Ulm, Germany.
Ulrich PannickeInstitute for Transfusion Medicine, Ulm University, Ulm, Germany.
Eva-Maria RumpInstitute for Transfusion Medicine, Ulm University, Ulm, Germany.
Hubert SchrezenmeierInstitute for Transfusion Medicine, Ulm University, Ulm, Germany.
Nicolas CasadeiInstitute of Medical Genetics and Applied Genomics, University of Tübingen, Tübingen, Germany.
Michaela PogodaInstitute of Medical Genetics and Applied Genomics, University of Tübingen, Tübingen, Germany.
Laurence KuhlburgerQuantitative Biology Center (QBiC), University of Tübingen, Tübingen, Germany.
Morgana Barroso OquendoQuantitative Biology Center (QBiC), University of Tübingen, Tübingen, Germany.
Stefan CzemmelQuantitative Biology Center (QBiC), University of Tübingen, Tübingen, Germany.
Klaus-Michael DebatinDepartment of Pediatrics and Adolescent Medicine, University Ulm Medical Center, Ulm, Germany.
Miriam ErlacherDepartment of Pediatrics and Adolescent Medicine, University Ulm Medical Center, Ulm, Germany.
Klaus SchwarzInstitute for Transfusion Medicine, Ulm University, Ulm, Germany.
Kerstin FelgentreffDepartment of Pediatrics and Adolescent Medicine, University Ulm Medical Center, Ulm, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: V(D)J recombination, initiated by recombination-activating gene (RAG) endonucleases, is a crucial process for the generation of diversified antigen receptors of T and B lymphocytes but regarded dispensable for innate natural killer (NK) lymphocytes lacking clonotypic receptors. Methods: To explore the impact of potential rearrangements on NK cell maturation, RAG-fate mapping reporter human induced pluripotent stem cell (iPSC) lines were generated by introduction of RSS-invEGFP constructs into the AAVS1 locus using CRISPR/Cas9 and differentiated into NK cells Results: GFP expression was observed in up to 14% of mature NK cells characterized by a CD45 Discussion: These findings suggest a role of RAG expression in NK cell ontogeny supporting the development of a terminally differentiated effector population.

Indexed as

Cell DifferentiationDNA-Binding ProteinsHomeodomain ProteinsKiller Cells, NaturalCRISPR-Cas SystemsHumansInduced Pluripotent Stem CellsV(D)J RecombinationDNA-Binding ProteinsHomeodomain ProteinsRAG-1 proteinDNA damage responseinduced pluripotent stem cellslymphoid progenitor cellsNK cell differentiationRAG-fate reporterRNA sequencingV(D)J recombination

Identifiers

PMID40787451
PMCPMC12331628

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.