ArticleInfection and drug resistance2025
Scopoletin and Scoparone from
Article in Infection and drug resistance, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
11 authors.
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Abstract
Purpose: Quorum sensing (QS) system is crucial for biofilm formation, virulence and survival of Materials and Methods: Compounds in VPE were identified using ultrahigh-performance liquid chromatography coupled with Q-exactive hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q Exactive-Orbitrap MS). Virulence factors of PAO1, including pyocyanin, rhamnolipids, elastase, hemolysin, swarming and swimming motility, and biofilm formation, were evaluated. Real-time reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect virulence-related genes. Bioassays for the QS inhibitory activity of VPE and its compounds were performed using Results: Sub-minimum inhibitory concentrations (sub-MICs) of VPE inhibited QS-related virulence factors in PAO1, including pyocyanin, rhamnolipids, biofilms, and swarming motility. Scopoletin and scoparone, two coumarin derivatives identified in VPE using UHPLC-Q Exactive-Orbitrap MS, also showed anti-QS activity against PAO1. These compounds reduced pyocyanin, rhamnolipid, and hemolysin production, elastase activity, biofilm formation and swarming motility. Further RT-qPCR assays revealed that scopoletin and scoparone universally suppress QS-associated genes ( Conclusion: Scopoletin and scoparone, two previously uncharacterized coumarin derivatives from VPE, were identified as novel QSIs. They are responsible for VPE's effect on weakening QS-controlled virulence of PAO1 by inhibiting QS-related genes. This study provides a laboratory basis for using scopoletin and scoparone as promising anti-virulence candidates for adjuvant therapy against
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