Evidence map›Paper›PMID 40785177›Full record

ArticleCurrent Alzheimer research2025

Neuroprotective Effects of Fenugreek Leaf Extract in a

Himanshi Varshney, Kajal Gaur, Iqra Subhan, Javeria Fatima, Smita Jyoti, Mantasha I, Mohd Shahid, Rahul, Yasir Hasan Siddique

Abstract read
PubMed Publisher
In one paragraph

Article in Current Alzheimer research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Biology · 2026
    Review
  2. Effect of Ascorbic Acid on the TransgenicCurrent Alzheimer research · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Himanshi VarshneyDepartment of Zoology, Faculty of Life Sciences, Laboratory of Alternative Animal Models, Section of Genetics, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Kajal GaurDepartment of Zoology, Faculty of Life Sciences, Laboratory of Alternative Animal Models, Section of Genetics, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Iqra SubhanDepartment of Zoology, Faculty of Life Sciences, Laboratory of Alternative Animal Models, Section of Genetics, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Javeria FatimaDepartment of Zoology, Faculty of Life Sciences, Laboratory of Alternative Animal Models, Section of Genetics, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Smita JyotiDepartment of Zoology, School of Sciences, IFTM University, Moradabad, Uttar Pradesh, India.
Mantasha IDepartment of Chemistry, Faculty of Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Mohd ShahidDepartment of Chemistry, Faculty of Sciences, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
RahulDepartment of Zoology, University of Allahabad, Prayagraj, Uttar Pradesh, India.
Yasir Hasan SiddiqueDepartment of Zoology, Faculty of Life Sciences, Laboratory of Alternative Animal Models, Section of Genetics, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionMuch emphasis has been given to the biological activities of Fenugreek against various diseased conditions. This study investigated the effect of fenugreek leaf extract on behavioural and cognitive function of transgenic Drosophila having human Aβ-42 expression in the neurons, herein referred as Alzheimer's disease model flies (AD flies).

methodsAD flies were exposed to four different doses of fenugreek leaf extract (FE) containing i.e., 0.005, 0.010, 0.015 and 0.02 g/ml for 30 days. Thereafter, behavioural and cognitive assessment was done using climbing ability, activity pattern, aversive phototaxis and odour choice indexes. The life span of different groups of flies was also recorded. The effect of FE on the oxidative stress markers, acetylcholinesterase, monoamine oxidase (MAO) and caspase 3 and 9 activities were determined. The deposition of Aβ-42 aggregates in the brain tissue of the flies was studied by performing immunostaining. Also, the metabolic profile of different groups of flies was studied by performing LC-MS/MS. Compared with control flies, 22 selected metabolites were found to be upregulated and downregulated among transgenic AD flies and FE exposed AD flies compared to control.

resultsThe findings of this study showed the neuroprotective role of fenugreek extract, which could be employed for the treatment of Alzheimer's disease. The AD flies exposed to FE showed a dose-dependent postponement in the decline of climbing ability, activity and cognitive impairments. A significant dose dependent increase in the life span was also noticed in the AD flies exposed to FE. A significant reduction in the oxidative stress, acetylcholinesterase, monoamine oxidase, and caspase-3&9 activities was also observed in a dose dependent manner. The results obtained from the immunostaining suggest the reduction in the deposition of Aβ-42 fibril, which was also confirmed by the docking studies showed the energetically favoured interaction useful for inhibiting the acetylcholinesterase and Aβ-42 aggregates. DISCUSSION: This study demonstrates the neurological potency of fenugreek leaf extract (FE) in a Drosophila model of AD due to its antioxidantive, anti-cholinesterase, and neuroprotective properties. Using a combination of behavioral, biochemical, histological, and metabolomic approaches, we evaluated the therapeutic potential of FE in mitigating AD-like symptoms in transgenic flies expressing Aβ-42.

conclusionFenugreek leaf extract may serve as a potential natural remedy for slowing down or alleviating the progression of AD.

Indexed as

Alzheimer DiseaseAmyloid beta-PeptidesNeuroprotective AgentsPeptide FragmentsPlant ExtractsTrigonellaAcetylcholinesteraseAnimalsAnimals, Genetically ModifiedBrainDisease Models, AnimalDose-Response Relationship, DrugDrosophilaDrosophila melanogasterHumansOxidative StressAcetylcholinesteraseAmyloid beta-Peptidesamyloid beta-protein (1-42)Neuroprotective AgentsPeptide FragmentsPlant ExtractsAlzheimer’s diseaseAβ-42Drosophilafenugreekhuman Aβ-42.metabolomicsoxidative stress

Identifiers

PMID40785177

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.