Evidence map›Paper›PMID 40784941›Full record

ArticleBMC microbiology2025

Biological characterization and genomic analysis of the newly discovered mycobacteriophage WST1.

Xingyan Tan, Xiaorui Zhang, Zhiqun He, Zhiping Luo, Ni An, Kerui Ye, Donghui Ke, Yuqing Li, Jumei Zeng

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Xingyan TanWest China School of Public Health and West China Fourth Hospital, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, China.
Xiaorui ZhangWest China School of Public Health and West China Fourth Hospital, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, China.
Zhiqun HeChengdu Center for Disease Control and Prevention, Chengdu, Sichuan, China.
Zhiping LuoState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, Sichuan, China.
Ni AnWest China School of Public Health and West China Fourth Hospital, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, China.
Kerui YeWest China School of Public Health and West China Fourth Hospital, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, China.
Donghui KeWest China School of Public Health and West China Fourth Hospital, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, China.
Yuqing LiState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, Sichuan, China.
Jumei ZengWest China School of Public Health and West China Fourth Hospital, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, 610041, China. zengjumei@scu.edu.cn.

Funding

the Fundamental Research Funds for the Central Universities YJ201985the National Base for International Science and Technology Cooperation of Chengdu University ARRLKF23-06the Sichuan Science and Technology Program 2023NSFSC1998the State Key Laboratory of Oral Diseases SKLOD-2025KP003
6 · The paper itself

Abstract

The rising prevalence of multidrug-resistant mycobacteria necessitates novel therapeutic strategies. Phages targeting clinically relevant mycobacteria remain scarce, lytic phages represent promising alternatives. In this study, we isolated mycobacterium phage WST1 from wastewater, exhibiting strict lytic specificity for Mycobacterium smegmatis. Genomic analysis revealed a 38,120 bp dsDNA genome (64.60% GC) with 59 ORFs, including tyrosine integrase, hicA/B toxin-antitoxin, but no virulence or resistance genes. WST1 demonstrated broad thermal stability (4–60℃) and pH (4–9) stability, but is UV-sensitive. Phylogenetically, WST1 formed a distinct clade with mycobacterium phage IdentityCrisis, the average nucleotide identity is 83.9%, WST1 possesses a terminase which shows 100% amino acid identity to IdentityCrisis, while its endolysin shows over 90% identity to other distant phages, showcasing the modular evolution of WST1.We discovered a new, safe phage candidate useful for both phage diversity studying and genetic engineering, thereby expanding the resources for mycobacteriophage therapeutic development.

Indexed as

Genome, ViralMycobacteriophagesDNA, ViralEndodeoxyribonucleasesEndopeptidasesGenomicsHost SpecificityHydrogen-Ion ConcentrationMycobacterium smegmatisOpen Reading FramesPhylogenySequence Analysis, DNAViral ProteinsDNA, ViralEndodeoxyribonucleasesendolysinEndopeptidasesterminaseViral ProteinsEndolysin-based treeMycobacteriophageMycobacterium smegmatisPhage biologyTerminase-based tree

Identifiers

PMID40784941
PMCPMC12337453

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.