ArticleEuropean journal of medical research2025
T cell-related diagnostic model and the underlying mechanism related to PRF1-mediated glycolysis in sepsis: evidences from single-cell, bulk transcriptomics, and experiment validation.
Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Identification of immune cell mitochondrial dysfunction characteristics and clinical predictive biomarkers in sepsis via multi-cohort machine learning and single-cell RNA sequencing.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026Article
- Bioinformatics analysis of pyroptosis-related differentially expressed genes in sepsis and diabetes mellitus.PloS one · 2026Article
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4 authors.
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Abstract
aimSepsis is a serious systemic inflammatory response. We aimed to construct a T cell-related diagnostic model for sepsis and uncover the underlying mechanisms.
methodsThrough downloading the single-cell RNA-sequencing (scRNA-seq) and RNA-seq data from online source, a series of bioinformatics methods including principal component analysis (PCA), differential expression analysis, and least absolute shrinkage and selection operator (LASSO) was used for selection of the T cell-related signatures. Then a diagnostic model was constructed and receiver operator characteristic (ROC) curve was used for evaluation of the diagnostic ability. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used for the function and pathways enrichment analysis. Metabolic flux analysis was performed to investigate the underlying metabolic mechanism. Finally, cecal ligation and puncture (CLP)-induced mouse sepsis model and LPS-induced RAW264.7 macrophage cell sepsis model were utilized to investigate the PRF1-mediated glycolysis mechanism in sepsis.
resultsscRNA-seq emphasized the important roles of T cell especially CD8
conclusionA 15-gene T cell-related diagnostic model for sepsis is constructed and PRF1 is confirmed to be an effective indicator and therapeutic target for sepsis, mainly functions in glycolysis.
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