Evidence map›Paper›PMID 40783745›Full record

ArticleBMC research notes2025

Comparison of the inhibitory effects of Lactobacillus supernatant and coculture on Gardnerella vaginalis.

Ferenc Somogyvári, Nándor Tűzkő, Attila Kereszturi, László Párducz, Mária Szécsényi, Valéria Endrész, Marianna Ábrók, Caleb M Ardizzone, Katalin Burián, Dezső Péter Virok

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ferenc SomogyváriDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis str. 6, Szeged, H-6725, Hungary.
Nándor TűzkőDepartment of Obstetrics and Gynecology, St. Margaret's Hospital, Budapest, H-1032, Hungary.
Attila KereszturiDepartment of Obstetrics and Gynecology, Albert Szent-Györgyi Health Center, Faculty of Medicine, University of Szeged, Semmelweis str. 1, Szeged, H-6701, Hungary.
László PárduczDepartment of Obstetrics and Gynecology, Pándy Kálmán County Hospital, Semmelweis str. 1, Gyula, H-5700, Hungary.
Mária SzécsényiDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis str. 6, Szeged, H-6725, Hungary.
Valéria EndrészDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis str. 6, Szeged, H-6725, Hungary.
Marianna ÁbrókDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis str. 6, Szeged, H-6725, Hungary.
Caleb M ArdizzoneDivision of Infectious Diseases, Department of Medicine, Indiana University School of Medicine, 635 Barnhill Drive, MS 258, Indianapolis, IN, 46202, USA.
Katalin BuriánDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis str. 6, Szeged, H-6725, Hungary.
Dezső Péter VirokDepartment of Medical Microbiology, Albert Szent-Györgyi Health Center and Albert Szent-Györgyi Medical School, University of Szeged, Semmelweis str. 6, Szeged, H-6725, Hungary. virok.dezso.peter@med.u-szeged.hu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveVaginal lactobacilli play a crucial role in inhibiting bacteria such as Gardnerella vaginalis (G. vaginalis), a key contributor to dysbiosis and bacterial vaginosis. We aimed to compare the inhibitory effects of Lactobacillus cell-free supernatants on G. vaginalis growth with those observed in Lactobacillus-G. vaginalis coculture, an experimental setup that more closely mimics in vivo conditions.

resultsTo identify the optimal medium for coculture experiments, MRS broth and NYC-III broth were compared. NYC-III significantly enhanced the growth of G. vaginalis and four of the five tested Lactobacillus strains. We then developed a direct quantitative PCR (qPCR) method that allowed us to specifically measure G. vaginalis genome concentrations in cocultures with Lactobacillus. This direct qPCR did not require DNA extraction and had a 4,096-fold dynamic range. We then assessed the inhibition of G. vaginalis growth by Lactobacillus cell-free supernatants in G. vaginalis cultures by measuring optical density and the Lactobacillus-mediated inhibition in cocultures by the G. vaginalis specific direct qPCR. The two measurement methods showed different levels of inhibitory activity for two of the five Lactobacillus strains tested. These findings suggest that coculture experiments should be conducted in place of, or in addition to, supernatant-based inhibitory assays.

Indexed as

Gardnerella vaginalisLactobacillusCoculture TechniquesCulture MediaFemaleHumansReal-Time Polymerase Chain ReactionVaginaVaginosis, BacterialCulture MediaAntimicrobialDirectDNAGardnerellaGrowthLactobacillusqPCRSupernatantVaginosis

Identifiers

PMID40783745
PMCPMC12335144

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.