Evidence map›Paper›PMID 40783509›Full record

ArticleBMC plant biology2025

CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit.

Xiaoyan Luo, Ying Dou, Yuxuan Lang, Huamei Zhao, Xinling Liu, Xiaoli Zhang, Yuxing Li, Dong Liang, Hui Xia

Abstract read
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xiaoyan Luo *College of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China.
Ying Dou *College of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China.
Yuxuan Lang *College of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China.
Huamei ZhaoCollege of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China.
Xinling LiuCollege of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China.
Xiaoli ZhangCollege of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China.
Yuxing LiCollege of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China.
Dong LiangCollege of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China. liangeast@sicau.edu.cn.
Hui XiaCollege of Horticulture, Sichuan Agricultural University, Huimin Road 211#, Chengdu, 611130, China. susanxia_2001@163.com.

Funding

Chengdu Science and Technology Department 2024-YF05-00408-SNNational Natural Science Foundation of China 32472679Sichuan Province Science and Technology Department Projects 2023YFN0095, 2024JDRC0011, 2025ZNSFSC0190
6 · The paper itself

Abstract

backgroundCRISPR/Cas9 technology has garnered increasing attention for its simplicity and precision in genome editing, making it an indispensable tool for gene function research and crop genetic improvement. However, the inefficiency and time-consuming nature of genetic transformation continue to pose substantial challenges to its widespread application in woody plants.

resultsIn this study, we developed a rapid and efficient Agrobacterium-mediated transformation system using petioles as explants for kiwifruit. Positive resistant seedlings were obtained within three months by inoculating on MS medium supplemented with 2.0 mg·L

conclusionsOverall, we established an efficient Agrobacterium-mediated transformation system using petioles as explants, which is applicable for CRISPR/Cas9-mediated gene editing in kiwifruit, thereby facilitating functional gene studies and genetic improvement.

Indexed as

ActinidiaCarotenoidsCRISPR-Cas SystemsAgrobacteriumBenzyl CompoundsFruitGene EditingGene Expression Regulation, PlantGenes, PlantGenome, PlantNaphthaleneacetic AcidsOxidoreductasesPlant Growth RegulatorsPlant ProteinsPlants, Genetically ModifiedPurinesbenzylaminopurineBenzyl CompoundsCarotenoidsNaphthaleneacetic AcidsOxidoreductasesphytoene dehydrogenasePlant Growth RegulatorsPlant ProteinsPurinesCRISPR/Cas9Genetic transformationKiwifruitPhytoene desaturase (PDS)ζ-carotene desaturase (ZDS)

Identifiers

PMID40783509
PMCPMC12335140

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.