Evidence map›Paper›PMID 40783493›Full record

ArticleNature communications2025

A multivalent mRNA vaccine elicits robust immune responses and confers protection in a murine model of monkeypox virus infection.

Yansheng Li, Lin Cheng, Lixu Jiang, Zhuohuan Li, Jing Rao, Tong Wu, Fangyan Zhang, Baocai Xie, Yu He, Lianrong Wang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yansheng Li *Department of Critical Care Medicine, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People' Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Lin Cheng *Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China.ORCID http://orcid.org/0000-0001-8066-527X
Lixu Jiang *Department of Critical Care Medicine, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People' Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.ORCID http://orcid.org/0000-0001-6800-7063
Zhuohuan LiInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China.
Jing RaoDepartment of Critical Care Medicine, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People' Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Tong WuDepartment of Critical Care Medicine, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People' Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Fangyan ZhangDepartment of Critical Care Medicine, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People' Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China.
Baocai XieDepartment of Respiratory Diseases, Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen, China.
Yu HeDepartment of Gastroenterology, Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
Lianrong WangDepartment of Respiratory Diseases, Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen, China. lianrong2008@gmail.com.ORCID http://orcid.org/0000-0003-1669-6340
Zheng ZhangInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China. zhangzheng1975@aliyun.com.ORCID http://orcid.org/0000-0002-3544-1389
Shi ChenDepartment of Critical Care Medicine, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Guangdong Provincial Key Laboratory of Systems Biology and Synthetic Biology for Urogenital Tumors, Shenzhen Institute of Translational Medicine, Shenzhen University Medical School, Shenzhen Second People' Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China. shichen@email.szu.edu.cn.ORCID http://orcid.org/0000-0002-8965-1015

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monkeypox virus (MPXV) has re-emerged globally since May 2022, posing a significant public health threat. To address this, we develop two multivalent mRNA vaccine candidates-AAL, encoding three MPXV antigens, and AALI, which combines AAL with an immune-enhancing IFN-α protein. Both vaccines are delivered via mannose-modified lipid nanoparticles to target dendritic cells. Here we show that these vaccines elicit strong antibody responses against vaccinia virus and multiple MPXV clades, induce robust memory B-cell and T-cell responses, and promote dendritic cell maturation. In mouse challenge models, both vaccines provide protection against clade IIb MPXV and vaccinia virus, significantly reducing viral loads and preventing lung damage. Immune profiling reveals enhanced B- and T-cell receptor diversity and distinct CDR3 motifs post-vaccination. These findings demonstrate the potential of using mRNA-based multivalent vaccines as an effective strategy for preventing mpox and related Orthopoxvirus infections.

Indexed as

Monkeypox virusMpox, MonkeypoxmRNA VaccinesViral VaccinesAnimalsAntibodies, ViralAntigens, ViralDendritic CellsDisease Models, AnimalFemaleMiceMice, Inbred C57BLNanoparticlesRNA, MessengerT-LymphocytesVaccines, SyntheticAntibodies, ViralAntigens, ViralmRNA VaccinesRNA, MessengerVaccines, SyntheticViral Vaccines

Identifiers

PMID40783493
PMCPMC12335498

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.