Evidence map›Paper›PMID 40783448›Full record

ArticleMikrochimica acta2025

Dual-signal multiplex assays based on non-tandem enzymes for simultaneous detection of multiple pathogenic bacteria.

Tianxin Xiang, Jin Wen, Jianhua Fang, Chuang Nie, Na Cheng, Ying Ying, Hongliang Tan

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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tianxin XiangJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, Jiangxi Medical Center for Critical Public Health Events, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, P.R. China.
Jin WenJiangxi Provincial Key Laboratory of Natural and Biomimetic Drugs Research, College of Pharmaceutical Science, Jiangxi Normal University, Nanchang, 330022, P.R. China.
Jianhua FangJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, Jiangxi Medical Center for Critical Public Health Events, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, P.R. China.
Chuang NieJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, Jiangxi Medical Center for Critical Public Health Events, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, P.R. China.
Na ChengJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, Jiangxi Medical Center for Critical Public Health Events, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, P.R. China.
Ying YingJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, Jiangxi Medical Center for Critical Public Health Events, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, P.R. China.
Hongliang TanJiangxi Provincial Key Laboratory of Prevention and Treatment of Infectious Diseases, Jiangxi Medical Center for Critical Public Health Events, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, P.R. China. hltan@jxnu.edu.cn.

Funding

National Natural Science Foundation of China 22064011Natural Science Foundation of Jiangxi Province 20242BAB26090
6 · The paper itself

Abstract

Enzymatic assays represent a promising alternative to conventional multiplex optical methods for multi-target detection due to their exceptional specificity and catalytic versatility. Nevertheless, most existing enzymatic systems are limited to single-target detection. In this work, we developed a dual-signal multiplex assay based on non-tandem enzymes for the simultaneous detection of multiple pathogenic bacteria. This assay utilizes the distinct catalytic activities of horseradish peroxidase (HRP) and invertase (INV) to produce two orthogonal signal outputs. Specifically, HRP catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine to produce a blue chromogenic signal measurable by absorbance, while INV hydrolyzes sucrose to yield glucose, which is quantified with a personal glucose meter. To implement this assay, HRP and INV are individually encapsulated within zeolitic imidazolate framework-90, and Fe

Indexed as

Bacteriabeta-FructofuranosidaseBiosensing TechniquesAnimalsBenzidinesColorimetryHorseradish PeroxidaseLimit of DetectionMagnetite NanoparticlesMilkZeolites3,3',5,5'-tetramethylbenzidineBenzidinesbeta-FructofuranosidaseHorseradish PeroxidaseMagnetite NanoparticlesZeolitesEnzymeMultiplex assayNon-tandem reactionPathogenic bacteria

Identifiers

PMID40783448

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.