Evidence map›Paper›PMID 40783340›Full record

ArticleJournal of cystic fibrosis : official journal of the European Cystic Fibrosis Society2025

Genomic and epidemiologic investigation of Mycobacterium abscessus isolates in a cystic fibrosis center to determine potential routes of transmission.

Jane E Gross, Jason Fullmer, Gregory McClelland, Silvia M Caceres, Katie R Poch, Nabeeh A Hasan, Fan Jia, L Elaine Epperson, Ettie M Lipner, Charmie K Vang and 6 more

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05686837 (Prospective Healthcare-Associated Links in Transmission of Nontuberculous Mycobacteria Among People With Cystic Fibrosis), which is not on this map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05686837 enrolling by invitationnot on this map

Prospective Healthcare-Associated Links in Transmission of Nontuberculous Mycobacteria Among People With Cystic Fibrosis

TypeobservationalSponsorUniversity of North Carolina, Chapel HillRan2022 to 2027Enrolled100ConditionsCystic Fibrosis, Nontuberculous Mycobacterial Pulmonary Infection
3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jane E GrossDepartment of Pediatrics, University of North Carolina, Chapel Hill, NC, USA; Department of Pediatrics, National Jewish Health, Denver, CO, USA; Department of Medicine, National Jewish Health, Denver, CO, USA. Electronic address: Jane_Gross@med.unc.edu.
Jason FullmerDepartment of Pediatrics, Dell Children's Medical Group, Austin, TX, USA.
Gregory McClellandDepartment of Pediatrics, Dell Children's Medical Group, Austin, TX, USA.
Silvia M CaceresDepartment of Pediatrics, University of North Carolina, Chapel Hill, NC, USA; Department of Medicine, National Jewish Health, Denver, CO, USA.
Katie R PochDepartment of Medicine, National Jewish Health, Denver, CO, USA.
Nabeeh A HasanCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, USA.
Fan JiaCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, USA.
L Elaine EppersonCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, USA.
Ettie M LipnerNational Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Charmie K VangCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, USA.
Jennifer R HondaDepartment of Cellular and Molecular Biology, University of Texas, Tyler, TX, USA.
Matthew J StrandDivision of Biostatistics, National Jewish Health, Denver, CO, USA.
Vinicius Calado Nogueira de MouraDivision of Mycobacterial and Respiratory Infections, National Jewish Health, Denver, CO, USA.
Charles L DaleyDivision of Mycobacterial and Respiratory Infections, National Jewish Health, Denver, CO, USA.
Michael StrongCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, USA.
Jerry A NickDepartment of Medicine, National Jewish Health, Denver, CO, USA.

Funding

Intramural NIH HHS Z99 AI999999
6 · The paper itself

Abstract

backgroundCystic Fibrosis (CF) Centers worldwide have reported healthcare-associated outbreaks of nontuberculous mycobacteria (NTM). We report a retrospective investigation of shared Mycobacterium abscessus strains among people with cystic fibrosis (pwCF) receiving care at Dell Children's/Ascension combined Pediatric and Adult CF Program (DCMC).

methodsWhole genome sequencing (WGS) was used to identify genetically similar isolates among 167 NTM isolates from 57 pwCF. Epidemiological investigation, respiratory and environmental isolate comparisons, and watershed mapping were performed.

resultsWGS analysis revealed four M. abscessus clusters, two ssp. abscessus and two ssp. massiliense. One subject was infected with two distinct clustered M. abscessus (ssp. abscessus and ssp. massiliense). Epidemiologic investigation demonstrated opportunities for healthcare-associated transmission within all clusters. Two ssp. massiliense subject pairs had healthcare overlaps and high genomic relatedness, including one cohabitating sibling pair. M. abscessus recovered from DCMC revealed genetic similarity to a respiratory isolate from one patient who was never exposed to the hospital environment.

conclusionsWe identified shared M. abscessus strains via genomic analysis among pwCF at DCMC. None of the clustered patient isolates matched hospital environmental isolates at the genomic level. One hospital environmental isolate had genomic similarity to a respiratory isolate of M. abscessus, but the epidemiologic investigation revealed no evidence of subject exposure to the hospital setting. One ssp. massiliense subject pair had the same level of pangenome relatedness as the sibling pair and epidemiological investigation revealed overlap in the clinic, supporting healthcare-associated person-to-person transmission among the pair within a cluster. One pwCF had polyclonal clustered infections, suggesting multiple environmental sources of acquisition outside the healthcare environment.

Indexed as

Cross InfectionCystic FibrosisMycobacterium abscessusMycobacterium Infections, NontuberculousAdultChildDisease OutbreaksFemaleHumansMaleRetrospective StudiesWhole Genome SequencingCystic fibrosisEpidemiologyHealthcare-associated infectionInfection acquisitionInfection transmissionMycobacterium abscessusNontuberculous mycobacteriaWhole genome sequencing

Identifiers

PMID40783340
PMCPMC12392799

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.