ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2025
Institutional Cost Savings and Improved Pathogen Detection by Replacing Sentinel Mice with Environmental Soiled Bedding Health Monitoring Methods.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Enhancing Rodent Welfare, Cost Savings, and Efficiency: Implementation and Review of Data-Driven Improvements.Journal of the American Association for Laboratory Animal Science : JAALAS · 2026Review
- Twenty years of health monitoring in a conventional neuroscience animal facility: challenges, strategies, and 3Rs-oriented approaches to animal welfare, personnel health, and research integrity.Frontiers in physiology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Use of soiled bedding sentinels (SBS) for rodent colony health monitoring is limited by inconsistent pathogen detection, reliance on live animals, high costs, and labor intensity. Sentinel-free soiled bedding (SFSB) offers a viable alternative for all rodent housing systems, overcoming limitations by using PCR testing of matrices exposed to soiled bedding. As an alternative, a matrix may be exposed to all cages via direct colony dredging (DCD). This study compared pathogen detection and costs between SFSB, DCD, and SBS for mice housed in individually-ventilated cage rack system cages. For each study rack, SFSB was performed with one matrix shaken in composite soiled bedding, while DCD was performed with a second matrix exposed to all soiled cages on the rack using a dredging method. We hypothesized that the SFSB and DCD matrices would detect Rodentibacter heylii, Rodentibacter pneumotropicus, Helicobacter typhlonius, Helicobacter mastomyrinus, Helicobacter hepaticus, Helicobacter bilis, Helicobacter rodentium, Helicobacter ganmani, and murine norovirus (MNV) with equal or superior efficacy to SBS, at a comparable or reduced program cost. All SBS failed to detect R. heylii, R. pneumotropicus, H. typhlonius, H. mastomyrinus, H. hepaticus, H. bilis, H. rodentium, and H. ganmani when tested by fecal PCR, and 25% failed to detect MNV when tested via serology. In contrast, SFSB and DCD matrices detected MNV, R. heylii, R. pneumotropicus, H. typhlonius, H. mastomyrinus, H. hepaticus, H. bilis, H. rodentium, and H. ganmani even with low pathogen prevalence, although neither method achieved 100% detection. DCD had negative ergonomic, workflow, and labor challenges compared with SFSB. Overall, SFSB and DCD had reduced costs and superior pathogen detection compared with SBS, while SFSB provided the most efficient and user-friendly approach for health monitoring by this institution.
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