Evidence map›Paper›PMID 40782045›Full record

ArticleNanomedicine (London, England)2025

Storage stability of cowpea mosaic virus cancer drug candidate - a two-year update.

Andrea Simms, Narek Minasov, Nicole F Steinmetz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomedicine (London, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Andrea SimmsAiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, CA, USA.
Narek MinasovAiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, CA, USA.
Nicole F SteinmetzAiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, CA, USA.

Funding

Resource for Biocomputing Visualization and InformaticsP41GM103311 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2012 to 2017
$8.2M
Dual-pronged nano-drug delivery using plant virus-like particles.R01CA224605 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Nicole Franziska Steinmetz · 2017 to 2026
$4.0M
Bioprinting Plant Virus Nanoparticles for Immunotherapy and Relapse Prevention of Ovarian CancerR01CA253615 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, FIERING, STEVEN · 2020 to 2024
$3.1M
Toward translation of a plant virus-based in situ vaccination nanotechnologyR01CA274640 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FIERING, STEVEN, RANJAN, ASHISH · 2022 to 2025
$2.5M
JEOL JEM-1400Plus Transmission Electron MicroscopeS10OD023527 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FARQUHAR, MARILYN GIST · 2017 to 2017
$579k
NCI NIH HHS R01 CA224605NCI NIH HHS R01 CA253615NCI NIH HHS R01 CA274640NIGMS NIH HHS P41 GM103311NIH HHS S10 OD023527
6 · The paper itself

Abstract

aimTo evaluate the structural stability and biological activity of cowpea mosaic virus (CPMV) after 2 years of storage. MATERIALS AND

methodsCPMV was stored at room temperature (RT), 4°C, and -20°C for 2 years. Structural stability was assayed using dynamic light scattering (DLS), size exclusion chromatography (SEC), native and denaturing gel electrophoresis, and transmission electron microscopy (TEM). Biological activity was assessed by anti-tumor efficacy studies using a B16F10 dermal mouse melanoma model and by a cowpea plant infection challenge.

resultsStorage at -20°C preserved CPMV's structural properties and biological activity. Storage at 4°C caused aggregation, S-protein cleavage, and RNA degradation - samples stored at 4°C had decreased anti-tumor efficacy yet retained plant infectivity. CPMV stored at RT was degraded and lost biological activity.

conclusionFrozen storage allows CPMV to maintain structural and biological stability for at least 2 years.

Indexed as

Antineoplastic AgentsComovirusAnimalsCell Line, TumorDrug StabilityDrug StorageHumansMelanoma, ExperimentalMiceVignaAntineoplastic Agentscancer immunotherapyCowpea mosaic virusintratumoral immunotherapystorage stabilitytranslational drug development

Identifiers

PMID40782045
PMCPMC12490393

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.