Evidence map›Paper›PMID 40781978›Full record

ArticleThe Journal of international medical research2025

Effect of sample vortex mixing on severe acute respiratory syndrome coronavirus 2 nucleic acid testing: Comparative analysis of individual samples and sample pools.

Guochen Jin, Binbin Yin, Peihao Wu, Xingjun Meng, Xiuzhi Duan, Jiancheng Qian, Bo Zhu

Abstract readComparative Study
In one paragraph

Article in The Journal of international medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Guochen JinDepartment of Laboratory Medicine, The Women's Hospital of Zhejiang University School of Medicine, China.
Binbin YinDepartment of Laboratory Medicine, The Women's Hospital of Zhejiang University School of Medicine, China.
Peihao WuDepartment of Laboratory Medicine, The Women's Hospital of Zhejiang University School of Medicine, China.
Xingjun MengDepartment of Laboratory Medicine, The Women's Hospital of Zhejiang University School of Medicine, China.ORCID 0000-0001-8855-1867
Xiuzhi DuanDepartment of Laboratory Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, China.
Jiancheng QianDepartment of Infectious Disease, Tongde Hospital of Zhejiang Province, China.
Bo ZhuDepartment of Laboratory Medicine, The Women's Hospital of Zhejiang University School of Medicine, China.ORCID 0000-0002-4298-8788

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ObjectiveThis study investigated the effect of sample vortex mixing on the cycle threshold (Ct) values of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid testing and further explored the underlying reasons for this effect.MethodsWe initially examined the impact of sample vortex mixing on Ct values. Subsequently, epithelial cells within the samples were quantified and stained using an improved Neubauer hemocytometer and Wright-Giemsa stain to elucidate the causative factors of Ct value changes. Finally, we expanded our sample size to validate the effect of vortex mixing on Ct values.ResultsWe observed a significant decrease in Ct values (all

Indexed as

COVID-19COVID-19 Nucleic Acid TestingRNA, ViralSARS-CoV-2Specimen HandlingEpithelial CellsHumansRNA, ViralCt valuereverse transcription–polymerase chain reactionsampleSevere acute respiratory syndrome coronavirus 2vortex mixing

Identifiers

PMID40781978
PMCPMC12335658

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.