Evidence map›Paper›PMID 40779792›Full record

ArticleACS infectious diseases2026

Rapid Detection of Carbapenemase-Producing Enterobacterales Using a Luminescent Whole-Cell Biosensor.

Mitchell A Jeffs, Shreyas Bhat, Josephine L Liu, Rachel A V Gray, Xena X Li, Prameet M Sheth, Christopher T Lohans

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Molecular Insights into Carbapenem Resistance inInternational journal of molecular sciences · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mitchell A JeffsDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Shreyas BhatDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Josephine L LiuDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Rachel A V GrayDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Xena X LiShared Hospital Laboratory, Toronto, Ontario M4N 3M5, Canada.
Prameet M ShethDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Christopher T LohansDepartment of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario K7L 3N6, Canada.ORCID 0000-0003-1094-0349

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Carbapenemase-producing Enterobacterales (CPE) pose an urgent global health threat due to their ability to inactivate carbapenems, a group of last-resort antibiotics. Infections caused by these pathogens are associated with poor patient outcomes, high mortality rates, and added burden to infection prevention and control programs, making early detection vital to ensure optimal antimicrobial therapy and appropriate implementation of infection control practices. In this study, we report the application of a luminescent whole-cell biosensor for the rapid detection of CPE. This biosensor provides positive test results within 2.5 h, inclusive of setup time, and has been validated with a panel of laboratory and clinical isolates producing a diverse range of carbapenemases (KPC, NDM, IMP, VIM, and OXA-48-like). The assay identified 81/83 CPE isolates tested with a sensitivity of 97.5%, including strains producing weak OXA-48-like carbapenemases, which are sometimes missed by currently used detection methods. The assay also demonstrated a specificity of 100%, with all non-CPE clinical isolates testing negative under the optimized assay conditions. Due to the rapid time-to-positivity, minimal setup requirements, and high sensitivity, this test could serve as an attractive alternative to CPE detection methods currently employed by clinical microbiology laboratories and could also facilitate CPE screening in other settings (

Indexed as

Bacterial Proteinsbeta-LactamasesBiosensing TechniquesCarbapenem-Resistant EnterobacteriaceaeEnterobacteriaceaeEnterobacteriaceae InfectionsAnti-Bacterial AgentsHumansLuminescent MeasurementsSensitivity and SpecificityAnti-Bacterial AgentsBacterial Proteinsbeta-Lactamasescarbapenemaseantibioticsantimicrobial resistancebiosensorcarbapenemase-producing Enterobacteralesdiagnostic assayinfectious disease

Identifiers

PMID40779792
PMCPMC12798771

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.