Evidence map›Paper›PMID 40779395›Full record

ArticleSTAR protocols2025

Protocol for characterizing biomolecular condensates through live-cell imaging and analysis.

Grant Richter, Cindy Maurel, Alison Hogan, Rowan A W Radford, Albert Lee, Roger S Chung, Marco Morsch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Grant RichterFaculty of Medicine, Health & Human Sciences, Macquarie Medical School, MND Research Centre, Macquarie University, Sydney, NSW 2109, Australia. Electronic address: grant.richter@mq.edu.au.
Cindy MaurelFaculty of Medicine, Health & Human Sciences, Macquarie Medical School, MND Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
Alison HoganFaculty of Medicine, Health & Human Sciences, Macquarie Medical School, MND Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
Rowan A W RadfordFaculty of Medicine, Health & Human Sciences, Macquarie Medical School, MND Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
Albert LeeFaculty of Medicine, Health & Human Sciences, Macquarie Medical School, MND Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
Roger S ChungFaculty of Medicine, Health & Human Sciences, Macquarie Medical School, MND Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
Marco MorschFaculty of Medicine, Health & Human Sciences, Macquarie Medical School, MND Research Centre, Macquarie University, Sydney, NSW 2109, Australia. Electronic address: marco.morsch@mq.edu.au.

Funding

In vivo characterization of the molecular drivers of biomolecular condensate formation in TDP-43 neuropathologyR21DA056320 · NIDA · MACQUARIE UNIVERSITY · PI MORSCH, MARCO · 2022 to 2023
$292k
NIDA NIH HHS R21 DA056320
6 · The paper itself

Abstract

Biomolecular condensates are important targets of investigation due to their physiological functions as well as their roles in neurodegenerative diseases, cancer, and drug delivery. Here, we present a protocol for characterizing biomolecular condensates across three confocal microscopy-based modalities, namely, subcellular localization, fluorescent recovery after photobleaching (FRAP), and time lapse analysis of mobility and fission/fusion events. We detail optimized image acquisition parameters and describe steps for analysis workflows utilizing ImageJ macros and plugins, including 2D and 3D analysis approaches.

Indexed as

Biomolecular CondensatesFluorescence Recovery After PhotobleachingImage Processing, Computer-AssistedHumansImaging, Three-DimensionalMicroscopy, ConfocalTime-Lapse ImagingCell-based AssaysCell BiologyMicroscopy

Identifiers

PMID40779395
PMCPMC12355527

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.