ArticleMicrobiology spectrum2025
Characteristics of four natural poliovirus type 1 variants with six-nucleotide deletion (2,783-2,788 nt) in the VP1 region.
Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the establishment of the Chinese acute flaccid paralysis (AFP) case surveillance system in 1999, around 7,200 strains of poliovirus (PV) type 1 have been identified and isolated. Among these, the VP1 region of 5,649 strains has been sequenced. Based on the existing VP1 region sequence library, four strains of type 1 PV with six-nucleotide deletion in the VP1 region, identified from AFP cases, healthy children, and environmental sewage samples, were identified, and their biological characteristics were investigated. Whole-genome sequence analysis showed that the similarity with the Sabin 1 strain was 99.5-99.8%, and the mutation rate in the VP1 region was only 0.11-0.55%, indicating that these strains are not vaccine-derived PVs. The missing nucleotide is located at positions 2,783-2,788 in the VP1 region, resulting in the deletion of amino acids 102 and 103 at neutralizing antigen site 1 (N-Ag I) in the BC loop. It is worth noting that the neutralization test results showed that the two strains detected from AFP and healthy children evaded immune recognition, whereas the other two from environmental sewage did not. Molecular docking and neutralization antigen site analyzes indicate that the deletion of nucleotides 2,783-2,788 in N-Ag I is not a critical factor leading to the development of neutralization escape variants.IMPORTANCEInterestingly, we observed that the VP3-60 mutation in N-Ag IIIa may be the main reason for the immune evasion of these two viruses. In addition, based on the temperature sensitivity experiments, the four viruses exhibited similar temperature sensitivity to the Sabin 1 strain and their replication ability at 39.5°C was comparable to vaccine-derived polioviruses. Although variants with six-nucleotide deletion (2,783-2,788 nt) in the VP1 region do not cause significant biological changes, they can still spread in the environment and among populations, posing a certain risk of transmission.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.