Evidence map›Paper›PMID 40778393›Full record

ArticleOptics communications2025

Photobleaching Analysis of Fluorescent Proteins in Two-Photon Microscopy at High Repetition Rates.

Chun Hung Weng, Kyu Young Han

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Optics communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Chun Hung WengCREOL, The College of Optics and Photonics, University of Central Florida, Orlando, Florida, USA.
Kyu Young HanCREOL, The College of Optics and Photonics, University of Central Florida, Orlando, Florida, USA.

Funding

Super-multiplexed fluorescence nanoscopy for imaging-based proteomicsR35GM138039 · NIGMS · UNIVERSITY OF CENTRAL FLORIDA · PI Kyu Young Han · 2020 to 2026
$2.2M
Multicolor two-photon excitation STED microscopy with multichannel compact semiconductor laserR21GM131163 · NIGMS · UNIVERSITY OF CENTRAL FLORIDA · PI DELFYETT, PETER, HAN, KYU YOUNG · 2019 to 2020
$382k
NIGMS NIH HHS R21 GM131163NIGMS NIH HHS R35 GM138039
6 · The paper itself

Abstract

Severe photobleaching in two-photon excitation fluorescence microscopy (TPM) has limited its potential for long-term and quantitative imaging of live cells and tissues. One solution is to excite fluorophores with a high-repetition rate which reduces the peak intensity of irradiance. However, there is a lack of knowledge about the general utility of this strategy for fluorescent proteins. Here, using simple photobleaching assay, we studied the photobleaching of EGFP and tdTomato at 80 MHz and 640 MHz repetition rates. Our analysis shows that the impact of high repetition rate excitation on reducing photobleaching in TPM is highly dependent on the excitation wavelength and fluorophores. Our results are useful for selecting optimal parameters to minimize photobleaching and photodamage in TPM.

Indexed as

EGFPfluorescent proteinhigh repetition ratephotobleachingtwo-photon microscopy

Identifiers

PMID40778393
PMCPMC12330829

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.