ArticleMolecular therapy. Methods & clinical development2025
Membrane adsorber design for lentiviral vector recovery.
Article in Molecular therapy. Methods & clinical development, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Fouling-Free Inertial Microfluidic Cell Clarification for Lentivirus Manufacturing.Biotechnology and bioengineering · 2026Article
- Downstream Workflows for Intermediate Purification of Lentiviral Vectors Using Tangential Flow Filtration and AEX Membrane Chromatography.Engineering in life sciences · 2025Article
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The growing demand for lentiviral vectors (LVs) in cell and gene therapies has highlighted significant challenges in large-scale LV production, particularly low product recovery. Anion-exchange chromatography (AIEX) is widely used for LV capture. However, AIEX accounts for most of the product loss in downstream processing, due to excessive interaction strengths in current commercially available membranes causing irreversible LV binding. To address this, a new AIEX membrane structure that reduces LV interaction strength by lowering ligand density, using direct ligand grafting, and incorporating tighter pore size distributions is tested. Four prototypes were created with Q and D anion-exchange chemistries at 1.05 (R1) and 1.32 (R2) μm pore sizes. Prototypes minimized product loss from irreversible binding, with high total particle recoveries irrespective of time spent in the adsorbed state (
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