Evidence map›Paper›PMID 40777428›Full record

ArticlebioRxiv : the preprint server for biology2025

Hedgehog and Bmp signaling pathways play opposing roles during establishment of the cardiac inflow tract in zebrafish.

Rhea-Comfort A Robertson, Hannah G Knight, Catherine Lipovsky, Jie Ren, Neil C Chi, Deborah Yelon

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Rhea-Comfort A RobertsonDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.
Hannah G KnightDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.
Catherine LipovskyDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.
Jie RenDivision of Cardiovascular Medicine, Department of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Neil C ChiDivision of Cardiovascular Medicine, Department of Medicine, University of California, San Diego, La Jolla, CA, 92093, USA.
Deborah YelonDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.ORCID 0000-0003-3523-4053

Funding

UCSD/SDSU IRACDAK12GM068524 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TREJO, JOANN · 2003 to 2025
$24.4M
GENETIC MECHANISMS AND REGULATIONT32GM007240 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAMPTON, RANDOLPH Y. · 1985 to 2019
$23.8M
TRAINING IN REPRODUCTIVE SCIENCEST32HD007203 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PAMELA L MELLON · 1988 to 2026
$4.8M
Genetic regulation of cardiac inflow tract formation in zebrafishR01HL158112 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHI, NEIL C, YELON, DEBORAH · 2021 to 2024
$2.0M
NHLBI NIH HHS R01 HL158112NICHD NIH HHS T32 HD007203NIGMS NIH HHS K12 GM068524NIGMS NIH HHS T32 GM007240
6 · The paper itself

Abstract

Cardiac pacemaking activity is controlled by specialized cardiomyocytes in the cardiac inflow tract (IFT), but the processes that determine IFT dimensions remain poorly understood. Here, we show that Hedgehog (Hh) signaling limits the number of IFT cardiomyocytes in the embryonic zebrafish heart. Inhibiting Hh signaling, either genetically or pharmacologically, results in an expanded IFT population. In contrast, reducing Bmp signaling decreases the number of IFT cardiomyocytes, while increasing Bmp signaling leads to an excess of IFT cardiomyocytes. Temporal inhibition of each pathway reveals that Hh and Bmp signaling act before myocardial differentiation to regulate IFT size. Simultaneous reduction of both Hh and Bmp signaling yields a relatively normal number of IFT cardiomyocytes, suggesting that these pathways function antagonistically during IFT development. Additionally, epistasis analysis suggests that Bmp signaling acts upstream of Wnt signaling to promote IFT formation, whereas Hh signaling limits IFT size in a Wnt-independent manner. Our results support a model in which Hh signaling restricts the establishment of the IFT progenitor pool, while Bmp signaling drives IFT progenitor specification prior to Wnt-directed IFT differentiation.

Indexed as

acvr1lcardiac specificationchordinIslet1pacemaker cellssmoothened

Identifiers

PMID40777428
PMCPMC12330708

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.