Evidence map›Paper›PMID 40777425›Full record

ArticlebioRxiv : the preprint server for biology2025

A high throughput assay to detect enzymatic polyethylene oxidation.

Ross R Klauer, Mekhi Williams, Darien K Nguyen, Megan Tarr, Dionisios G Vlachos, Kevin V Solomon, Mark A Blenner

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Ross R KlauerChemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0000-0001-9638-3613
Mekhi WilliamsChemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0009-0000-7736-912X
Darien K NguyenChemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0000-0001-7204-1467
Megan TarrChemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0009-0007-4216-693X
Dionisios G VlachosChemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0000-0002-6795-8403
Kevin V SolomonChemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0000-0003-2904-9118
Mark A BlennerChemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.ORCID 0000-0001-9274-3749

Funding

Chemistry-Biology Interface Predoctoral Training Grant 2024-2029T32GM133395 · NIGMS · UNIVERSITY OF DELAWARE · PI Catherine Leimkuhler Grimes · 2019 to 2026
$3.7M
NIGMS NIH HHS T32 GM133395
6 · The paper itself

Abstract

Biological plastics deconstruction and upcycling have emerged as a sustainable alternative to traditional recycling technologies for plastics waste. The discovery and engineering of efficient thermostable poly(ethylene terephthalate) (PET) hydrolases has made biological PET recycling possible at scale; however, enzymes for non-PET plastics, which account for approximately 70% of all plastics produced, remain largely undiscovered. To accelerate the discovery of such enzymes, a high throughput screening (HTS) platform is needed. Here, we develop a HTS liquid-based assay to detect one of the first committed steps of polyolefin degradation, oxidation of the C-H bond to an aldehyde. We test 4-hydrazino-7-nitro-2,1,3-benxoxadiozole hydrazine (NBD-H), which reacts with generated aldehydes to form a fluorescent hydrazone, on oxidized low-density polyethylene (LDPE) films. Hydrazone generation correlated well with established carbonyl index metrics for polymer oxidation (R

Indexed as

biocatalysisbiodegradationhigh-throughputplasticsscreening

Identifiers

PMID40777425
PMCPMC12330763

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.