Evidence map›Paper›PMID 40777384›Full record

ArticlebioRxiv : the preprint server for biology2025

Capillary constrictions prime cancer cell tumorigenicity through PIEZO1.

G Silvani, C Kopecky, S Romanazzo, V Rodriguez, A Das, E Pandzic, J G Lock, C Chaffer, K Poole, K A Kilian

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

G SilvaniSchool of Materials Science and Engineering, UNSW Sydney, Australia.
C KopeckySchool of Chemistry, Australian Centre for NanoMedicine (ACN), UNSW Sydney, Australia.
S RomanazzoSchool of Chemistry, Australian Centre for NanoMedicine (ACN), UNSW Sydney, Australia.
V RodriguezGarvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia.
A DasSchool of Biomedical Sciences, Faculty of Medicine and Health, UNSW Sydney, Australia.
E PandzicKatharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, UNSW, Sydney, Australia.
J G LockSchool of Biomedical Sciences, Faculty of Medicine and Health, UNSW Sydney, Australia.
C ChafferGarvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia.
K PooleSchool of Biomedical Sciences, Faculty of Medicine & Health, UNSW Sydney, Australia.
K A KilianSchool of Materials Science and Engineering, UNSW Sydney, Australia.ORCID 0000-0002-8963-9796

Funding

Engineered microtumor arrays for development of combination therapiesR01CA251443 · NCI · UNIVERSITY OF NEW SOUTH WALES · PI COPLAND, JOHN A., KILIAN, KRISTOPHER ALAN · 2020 to 2024
$998k
NCI NIH HHS R01 CA251443
6 · The paper itself

Abstract

Metastasis is a hallmark of cancer and is responsible for the majority of cancer-related deaths. Evidence suggests that even a single cancer cell can spread and seed a secondary tumour. However, not all circulating tumour cells have this ability, which implies that dissemination and distal growth require adaptative mechanisms during circulation. Here we report that constriction during microcapillary transit will trigger reprogramming of melanoma cells to a tumorigenic cancer stem cell-like state. Using a microfluidic device mimicking physiological flow rates and gradual capillary narrowing, we showed that compression through narrow channels lead to cell and nuclear deformation, rapid changes in chromatin state and increased calcium handling through the mechanosensor PIEZO1. Within minutes of microcapillary transit, cells show increased regulation of transcripts associated with metabolic reprogramming and metastatic processes, which culminates in the adoption of cancer stem cell-like properties. Squeezed cells displayed elevated melanoma stem cell markers, increased propensity for trans-endothelium invasion, and characteristics of enhanced tumorigenicity

Identifiers

PMID40777384
PMCPMC12330622

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.