Evidence map›Paper›PMID 40777344›Full record

ArticlebioRxiv : the preprint server for biology2025

Primary bovine embryonic fibroblasts support seasonal influenza A virus infection and demonstrate variable fitness of HPAI H5N1.

Grace K Wenger, Deann T Snyder, Justin R Prigge, Allyson H Turner, Sara A Jaffrani, Edward E Schmidt, Emily A Bruce, Emma K Loveday

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Grace K WengerMicrobiology and Cell Biology Department, Montana State University, Bozeman, MT, United States.ORCID 0009-0000-6616-2502
Deann T SnyderMicrobiology and Cell Biology Department, Montana State University, Bozeman, MT, United States.ORCID 0000-0003-3087-7260
Justin R PriggeMicrobiology and Cell Biology Department, Montana State University, Bozeman, MT, United States.ORCID 0000-0003-4666-0728
Allyson H TurnerDepartment of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT, United States.ORCID 0009-0009-9576-6095
Sara A JaffraniDepartment of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT, United States.
Edward E SchmidtMicrobiology and Cell Biology Department, Montana State University, Bozeman, MT, United States.ORCID 0000-0002-9959-4990
Emily A BruceDepartment of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT, United States.ORCID 0000-0001-8391-370X
Emma K LovedayMicrobiology and Cell Biology Department, Montana State University, Bozeman, MT, United States.ORCID 0000-0002-1154-7728

Funding

Using Dengue Controlled Human Infection Model to Identify Adaptive Immune Correlates of ProtectionP20GM125498 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI Kristen Pierce · 2018 to 2026
$24.9M
Single cell heterogeneity of influenza A virus genetic diversity and host adaptation using drop-based microfluidicsR21AI178432 · NIAID · MONTANA STATE UNIVERSITY - BOZEMAN · PI LOVEDAY, EMMA KATE · 2023 to 2024
$421k
NIAID NIH HHS R21 AI178432NIGMS NIH HHS P20 GM125498
6 · The paper itself

Abstract

The emergence of highly pathogenic avian influenza (HPAI) H5N1 (clade 2.3.4.4b, genotype B3.13) in dairy cattle presents substantial challenges to the agricultural sector and public health. Mechanistic studies of infection and transmission in cattle have proven difficult due to animal handling restrictions as well as limited availability of established cell culture models. Primary Bovine Embryonic Fibroblasts (BeEFs) were collected from a Montana cow and are investigated here as a model to study influenza A virus (IAV) infection dynamics. We compared sialylation profiles, infectious virus production, viral replication, and plaque morphology in both BeEFs and chicken DF-1 cells following infection with the bovine HPAI H5N1 and an earlier 2.3.4.4b genotype (B1.1) isolated in 2022. The data presented here show increased viral fitness of the bovine origin HPAI H5N1 strains across multiple species and bovine susceptibility to human seasonal IAV. This study highlights the ability of BeEFs to serve as a model for studying IAV infections in bovine hosts.

Identifiers

PMID40777344
PMCPMC12330616

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.