ArticleThe plant pathology journal2025
Inhibition of Hop Stunt Viroid by Exogenous Double-Stranded RNA in Micropropagated Grapevine Plantlets.
Article in The plant pathology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Rapid and visual detection of Lentinula edodes spherical virus using CRISPR/Cas12a-based RT-RPA.Virus genes · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Hop stunt viroid (HSVd) infection reduces grapevine (Vitis vinifera) yield and quality, leading to significant economic losses. Conventional methods for producing virus-free plantlets often fail to completely eliminate viroids, necessitating alternative strategies. In this study, RNA interference (RNAi) was induced by applying HSVd-specific double-stranded RNA (dsRNA) to infected grapevine plantlets. Exogenous dsRNA treatment significantly reduced HSVd levels, as confirmed by reverse transcription polymerase chain reaction and digital PCR. Fluorescently labeled (Cy3) dsRNA uptake was detected in plant tissues, while small RNA sequencing revealed an accumulation of HSVd-derived small interfering RNA, indicating RNAi activation. Notably, the inhibitory effect persisted through three successive generations without additional treatment, and similar suppression was observed in HSVd-infected cucumber plants. These findings highlight the efficacy and durability of exogenous dsRNA applications as a sustainable and non-transgenic approach for viroid control in grapevine cultivation.
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