Evidence map›Paper›PMID 40775476›Full record

ArticleBioprocess and biosystems engineering2025

From wheat straw to soluble yeast extract: enhanced mycoprotein production by adaptively evolved Trichosporon cutaneum and fermentation pH shifting.

Chaolong Qu, Dayu Ren, Qi Liu, Bin Zhang, Jie Bao

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Article in Bioprocess and biosystems engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Chaolong QuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Dayu RenState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Qi LiuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.
Bin ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. binzh@ecust.edu.cn.
Jie BaoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China. jbao@ecust.edu.cn.

Funding

Key Technology R&D Program of the Science and Technology Commission of Shanghai Municipality 25HC2820200National Key R&D Program of China 2023YFA0914400National Natural Science Foundation of China 32301269Yangfan Project of Science and Technology Committee of Shanghai Municipality 23YF1409900
6 · The paper itself

Abstract

Single-cell protein (SCP) produced by yeast using low-cost agricultural wastes shows great potential as an alternative protein source for animal and human nutrition. In this study, we developed an adaptive evolution method coupled with centrifugal fractionation and pH shifting to enhance SCP production by Trichosporon cutaneum from wheat straw. During the adaptive evolution, the culture pH was shifted from 5.0 to 7.0, which is more favorable for SCP accumulation of T. cutaneum. The finally obtained T. cutaneum CL160 exhibited a 109.2% increase in SCP content compared to the parental strain. The DCW and SCP titer of T. cutaneum CL160 reached 48.6 ± 1.5 g/L and 14.2 ± 1.1 g/L using wheat straw clarified hydrolysate by batch fermentation. Fed-batch fermentation using wheat straw-derived syrup further improved DCW and SCP titer to 124.2 g/L and 32.6 g/L. Further attempts were performed to prepare soluble yeast extract from lignocellulose-derived SCP by cell autolysis. This yeast extract served as an effective nitrogen source for lactic acid fermentation by Pediococcus acidilactici, achieving 83.2 ± 1.1 g/L lactic acid titer and 45 × 10

Indexed as

FermentationFungal ProteinsTrichosporonTriticumDietary ProteinsHydrogen-Ion ConcentrationDietary ProteinsFungal Proteinssingle cell proteinsAdaptive evolutionLignocellulosepH shiftingSingle cell proteinYeast extract

Identifiers

PMID40775476

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.