Evidence map›Paper›PMID 40775356›Full record

ArticleBMC veterinary research2025

Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry.

Parichart Tesena, Ruethaiwan Vinijkumthorn, Amornthep Kingkaw, Petchpailin Yanyongsirikarn, Khajornpol Phasuk, Sekkarin Ploypetch, Narumon Phaonakrop, Sittiruk Roytrakul, Wanwipa Vongsangnak, Nawarus Prapaiwan

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Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Parichart TesenaDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Ruethaiwan VinijkumthornDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Amornthep KingkawFaculty of Science, Interdisciplinary Graduate Program in Bioscience, Kasetsart University, Bangkok, 10900, Thailand.
Petchpailin YanyongsirikarnFaculty of Veterinary Science, Equine Clinic, Prasuarthon Small Animal Hospital, Mahidol University, Nakhon Pathom, 73170, Thailand.
Khajornpol PhasukVeterinary Medicine Disposal and Animal Husbandry, Sub-Division Patrol Special Operation Division, Mounted Police Sub-Division, Bangkok, 10150, Thailand.
Sekkarin PloypetchDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand.
Narumon PhaonakropFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
Sittiruk RoytrakulFunctional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
Wanwipa VongsangnakDepartment of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand. wanwipa.v@ku.ac.th.
Nawarus PrapaiwanDepartment of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, 73170, Thailand. nawarus.pra@mahidol.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEquine melanocytic neoplasm (EMN) is a skin tumor commonly observed in grey horses. Limited research has yet to investigate proteomic profiles of EMN, particularly in the early stages and their expression patterns. This study, therefore, aimed to identify signature proteins from tissue biopsies to distinguish early EMN, severe EMN, and normal groups.

resultsUsing proteomic analysis of 19 tissue samples (normal: n = 6, early EMN: n = 7, severe EMN: n = 6) through LC-MS/MS, 12,310 proteins were identified. Differentially expressed proteins (DEPs) and functional interaction analysis revealed significant overexpression of Wnt signature proteins, e.g., canonical (Wnt2B) and non-canonical (Wnt5B) Wnt signaling in early EMN stages. Immunohistochemical staining (IHC) towards immunolocalizing Wnt signature protein, particularly the Wnt2B functional signal, further verified its higher expression in early EMN compared to other groups.

conclusionsThese findings suggest that the Wnt pathway and functional insight are key mediators in signal transduction during early EMN, offering potential markers for initial stage detection. This study enhances the understanding of EMN mechanisms and the role of Wnt proteins, with implications for developing future diagnostic and therapeutic strategies.

Indexed as

Horse DiseasesMelanomaSkin NeoplasmsWnt Signaling PathwayAnimalsFemaleHorsesImmunohistochemistryMaleProteomicsTandem Mass SpectrometryEquine melanocytic neoplasmGrey horsesImmunohistochemistryProteomicsWnt pathway

Identifiers

PMID40775356
PMCPMC12329947

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