Evidence map›Paper›PMID 40775118›Full record

ArticleMolecular biology reports2025

Systematic identification, evolution and expression analyses of Brachypodium distachyon NHX gene family in response to salinity.

Hatem Boubakri, Siwar Messaoudi, Mhemmed Gandour

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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2 citing papers in PubMed.

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5 · Who and what money

Authors and funding

3 authors.

Hatem BoubakriLaboratory of Legumes and Sustainable Agrosystems, Centre of Biotechnology of Borj-Cedria, Hammam-Lif, B.P 901, 2050, Tunisia. hatem.boubakri@cbbc.rnrt.tn.
Siwar MessaoudiLaboratory of Legumes and Sustainable Agrosystems, Centre of Biotechnology of Borj-Cedria, Hammam-Lif, B.P 901, 2050, Tunisia.
Mhemmed GandourLaboratory of Extremophile Plants, Centre of Biotechnology of Borj- Cedria, Hammam-Lif, Tunisia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSalinity stress is a major abiotic factor affecting plant cultivation and productivity worldwide. Brachypodium distachyon emerged as a model for understanding stress adaptation mechanisms in grasses. METHODS AND

resultsSodium/Hydrogen antiporter gene (NHX) family in B. distachyon (BdNHX) was systemically identified, characterized and their expression pattern was assessed in response to salinity by Real Time q-PCR. Overall, 8 NHX genes were identified in the B. distachyon genome (BdNHX1-8). Phylogenetic, motifs and gene structure analyses allowed the classification of BdNHX genes into 3 groups as a function of their subcellular localization: Vacuole (Vac-Class), endosomal (Endo-Class) and plasma membrane (PM-Class). Protein-protein interactions (PPIs) network determination indicated that several BdNHX proteins interacts with CIPK24, CBL4 and KEA4, implying their involvement in CBL-CIPK pathway. Expression studies using Real Time quantitative-PCR (RT q-PCR) revealed the upregulation of specific BdNHX genes under salinity in both leaves and roots, suggesting their involvement in salinity tolerance mechanisms in B. distachyon.

conclusionThese results provided valuable targets of B. distachyon NHX members for genetic engineering to improve grasses resilience to salinity.

Indexed as

BrachypodiumSodium-Hydrogen ExchangersEvolution, MolecularGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantMultigene FamilyPhylogenyPlant ProteinsPlant RootsProtein Interaction MapsSalinitySalt StressSalt ToleranceStress, PhysiologicalPlant ProteinsSodium-Hydrogen ExchangersAntiporterBrachypodium distachyonGene expressionIon transporterNHX gene familySalinity

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