Evidence map›Paper›PMID 40775117›Full record

ArticleMolecular and cellular biochemistry2025

PACS2/PKCα/NOX4 pathway damaged the renal vascular endothelial barrier by promoting ROS production in diabetic nephropathy mice.

Xinru Zheng, Qianjun Zhu, Jie Ouyang, Jing Zhang, Quanjun Liu, Jianing Fan, Peng Gao, Haijiao Long, Hong Xiang, Hongwei Lu

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Article in Molecular and cellular biochemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Letter to the Editors-correspondence.Molecular and cellular biochemistry · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xinru Zheng *Health Management Center, The Third Xiangya Hospital of Central South University, Changsha, China.
Qianjun Zhu *Department of Cardiothoracic Surgery, The Third Xiangya Hospital of Central South University, Changsha, China.
Jie OuyangHealth Management Center, The Third Xiangya Hospital of Central South University, Changsha, China.
Jing ZhangHealth Management Center, The Third Xiangya Hospital of Central South University, Changsha, China.
Quanjun LiuHealth Management Center, The Third Xiangya Hospital of Central South University, Changsha, China.
Jianing FanHealth Management Center, The Third Xiangya Hospital of Central South University, Changsha, China.
Peng GaoHealth Management Center, The Third Xiangya Hospital of Central South University, Changsha, China.
Haijiao LongHealth Management Center, The Third Xiangya Hospital of Central South University, Changsha, China.
Hong XiangCenter for Experimental Medicine, The Third Xiangya Hospital of Central South University, 138 Tongzipo Road, Changsha, 410013, Hunan, China. xianghong168@csu.edu.cn.
Hongwei LuHealth Management Center, The Third Xiangya Hospital of Central South University, Changsha, China. hongweilu@csu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Renal vascular endothelial barrier dysfunction plays an important role in the pathogenesis of diabetic nephropathy (DN). Reactive oxygen species (ROS) contribute to barrier dysfunction in various aspects of diabetes. Phosphofurin acidic cluster sorting protein 2 (PACS2) is related to the ROS production, but the specific signaling pathway in endothelial cells remains unclear. In this study, we explored the mechanistic function of PACS2 and its downstream PKCα/NOX4 signaling pathway in endothelial barrier damage in DN. A significant upregulation of PACS2 expression was observed in human umbilical vein endothelial cells treated with high glucose and palmitic acid and glomerular endothelial cells derived from STZ + HFD-induced DN mice. SiRNA-mediated silencing or knockdown of PACS2 reversed the impaired vascular barrier function in vivo and in vitro. Furthermore, the inhibition of PACS2 significantly downregulated the protein expression of PKCα and NOX4 protein and the production of ROS in endothelial cells. Collectively, our findings indicate that the PACS2/PKCα/NOX4 signaling pathway may participate in the pathogenesis of DN by regulating vascular endothelial barrier function.

Indexed as

Adaptor Proteins, Signal TransducingDiabetes Mellitus, ExperimentalDiabetic NephropathiesEndothelium, VascularNADPH Oxidase 4Protein Kinase C-alphaReactive Oxygen SpeciesSignal TransductionVesicular Transport ProteinsAnimalsHumansHuman Umbilical Vein Endothelial CellsMaleMiceMice, Inbred C57BLAdaptor Proteins, Signal TransducingNADPH Oxidase 4Nox4 protein, mousePrkca protein, mouseProtein Kinase C-alphaReactive Oxygen SpeciesVesicular Transport ProteinsDiabetic nephropathyEndothelial barrier functionPhosphofurin acidic cluster sorting protein 2Reactive oxygen species

Identifiers

PMID40775117

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.