Transcriptomic profiling of canine mammarygland tumors reveals subtype-specific and shared differentially expressed mRNAs.

backgroundCanine mammary gland tumors (MGTs) are among the most frequently diagnosed neoplasms in intact female dogs and are increasingly regarded as a potential model of human breast cancer. However, the transcriptomic landscape across distinct histological subtypes remains poorly characterized. To explore the molecular alterations underlying MGT subtypes and to identify potential biomarkers, we performed transcriptomic profiling using next-generation sequencing (NGS). METHOD AND

resultA total of 18 canine MGT samples were analyzed using NGS. mRNA expression levels were compared between three groups: dogs with benign mixed tumor, adenoma, or carcinoma, and healthy controls. DEGs were identified from NGS reads with the Empirical Analysis of DGE tool (CLC genomic workbench) and subjected to functional enrichment analysis. A total of 47 DEGs were identified across MGT subtypes. Among these, two genes, overexpressed gene ENSCAFG00845029941 and underexpressed gene FGL2, were selected for validation via quantitative RT-PCR based on statistical significance and biological relevance. DEG clustering was observed on chromosomes 20 (carcinoma group) and 26 (benign mixed tumor and adenoma groups). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed enrichment in pathways related to muscle development and calcium ion binding.

conclusionWe potentially implicated FGL2 and ENSCAFG00845029941 as DEGs involved in multi-subtype oncogenesis for canine MGTs, as novel findings. DEGs on chromosome 26 may be implicated in benign tumor oncogenesis, and those on chromosome 20 may be similarly implicated in carcinoma.