Evidence map›Paper›PMID 40773493›Full record

ArticleMolecular plant pathology2025

MdPOB1L Positively Regulates Disease Resistance Against Botryosphaeria dothidea by Manipulating MdNPR1 Protein Stability in Malus domestica.

Fujun Zhang, Haojian Li, Xinsong Guo, Ping Sun, Ning Ma, Lianzhen Li, Mingxi Xuan, Zhangji Luo, Yi Tian, Chunxiang You and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular plant pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Fujun ZhangCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Haojian LiCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Xinsong GuoKey Laboratory of Humic Acid Fertilizer, Ministry of Agriculture, Shandong Agricultural University Fertilizer Science Tech. Co Ltd., Tai-An, Shandong, China.
Ping SunCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Ning MaCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Lianzhen LiCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Mingxi XuanCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Zhangji LuoCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Yi TianCollege of Horticulture, Hebei Agricultural University, Baoding, China.
Chunxiang YouCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.
Zhenlu ZhangCollege of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement, Shandong Agricultural University, Tai-An, Shandong, China.ORCID 0000-0002-5507-896X

Funding

National Key Research and Development Program of China 2022YFD1201700National Natural Science Foundation of China 32072524National Natural Science Foundation of China 32172538Natural Science Foundation of Shandong Province ZR2024MC225
6 · The paper itself

Abstract

E3 ubiquitin ligase is a key component of the ubiquitin-proteasome system, which is deeply involved in multiple aspects of plant growth and development, including in plant defence responses. POZ/BTB containing-protein1 (POB1) is a type of BTB-BACK domain-containing E3 ligase, which was previously reported to be a negative regulator of defence responses in multiple plant species. In this report, we identified MdPOB1-like (MdPOB1L) as a positive regulator in defence responses against Botryosphaeria dothidea by manipulating protein stability of MdNPR1, a master regulator in salicylic acid (SA) signalling pathway, in apple (Malus domestica). We first found that MdPOB1L is evolutionarily close to PbPOB1 from Pyrus bretschneideri, and it was localised in the nucleus in the epidermal cells of Nicotiana benthamiana leaves. Reverse transcription-quantitative PCR analysis revealed that MdPOB1L was inducible upon B. dothidea infection or SA treatment. Further investigation demonstrated that overexpressing MdPOB1L enhanced disease resistance to B. dothidea in both apple calli and fruits, while repressing its transcription displayed the opposite phenotype. Moreover, overexpressing MdPOB1L promoted the transcription of SA-responsive pathogenesis-related (PR) genes and MdNPR1. We further demonstrated that MdPOB1L interacted with MdNPR1 in the nucleus and promoted its ubiquitination and degradation through the proteasome pathway, which might contribute to the turnover of MdNPR1 in the nucleus, leading to enhanced downstream responses of the SA signalling pathway. Therefore, we demonstrate here that MdPOB1L positively regulates defence responses against B. dothidea by manipulating MdNPR1 protein stability in apple.

Indexed as

AscomycotaDisease ResistanceMalusPlant DiseasesPlant ProteinsGene Expression Regulation, PlantNicotianaPlants, Genetically ModifiedProtein StabilitySalicylic AcidPlant ProteinsSalicylic AcidBotryosphaeria dothideaMdNPR1MdPOB1Lprotein ubiquitination and degradationsalicylic acid signalling pathway

Identifiers

PMID40773493
PMCPMC12330943

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.