Evidence map›Paper›PMID 40773093›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2025

Chlorophyll deficient 5 encodes a DNA helicase and is essential for chloroplast development in rice.

Weichi Liu, Xiaonan Han, Jiaxin Wei, Jie Yang, Yingchun Wan, Hongxia Yang, Yao Deng, Nan Wang, Ping Feng, Wenqiang Shen

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Weichi Liu *Rice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Xiaonan Han *Rice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Jiaxin Wei *Rice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Jie YangRice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Yingchun WanRice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Hongxia YangRice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Yao DengRice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Nan WangRice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China. wangnan_xndx@126.com.
Ping FengRice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China. fengpingfighting@163.com.
Wenqiang ShenRice Research Institute, Key Laboratory of Crop Molecular Improvement, Engineering Research Center of South Upland Agriculture, Ministry of Education, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China. wqshen@swu.edu.cn.ORCID http://orcid.org/0000-0001-9132-3160

Funding

Fuling Research Institute of Southwest University Co-construction Project FLYJY202402Innovative Research Group Project of the National Natural Science Foundation of China cstc2022ycjh-bgzxm0025Sichuan Science and Technology Program 2024NSFJQ0031
6 · The paper itself

Abstract

key messageA novel gene CDE5 controlling leaf color was identified. Its causal gene LOC_Os06g45980 was verified by complementation transgenic, expression analysis, RNA sequencing, enzyme activity tests. Leaf color is a critical trait influencing plant biosynthesis, as it directly impacts photosynthesis and dry matter production. Despite intensive research, the molecular mechanisms of leaf color still remain to be elucidated. In the present study, we characterized a rice chlorophyll deficient 5 (cde5) mutant, which was defective in chloroplast development and exhibited albino leaves throughout the overall growth period. Map-based cloning revealed that CDE5 encodes a DNA helicase which has not been previously reported to be associated with albino leaf in plants. The cde5 mutant exhibited abnormal chloroplast structures and decreased chlorophyll contents. Further expression analyses showed that CDE5 expressed in variety of tissues, and in situ hybridization results showed CDE5 expressed in mesophyll cell. Subcellular localization analysis demonstrated that CDE5 was localized in the chloroplast and mitochondrion. RNA sequencing and RT-qPCR showed that several genes involved in chlorophyll synthesis, chloroplast development and photosynthesis were significantly altered due to loss of CDE5. Enzyme activity experiments further demonstrated that CDE5 exhibits ATPase activity, while the ATPase activity of the cde5 mutant protein is significantly reduced. Taken together, these findings suggest that CDE5 plays an important role in leaf color regulation, thus shedding new light on the function of DNA helicase in rice.

Indexed as

ChlorophyllChloroplastsDNA HelicasesOryzaPlant ProteinsGene Expression Regulation, PlantGenes, PlantMutationPhenotypePhotosynthesisPlant LeavesChlorophyllDNA HelicasesPlant Proteins

Identifiers

PMID40773093

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.