Preparation and identification of a novel monoclonal antibody against African swine fever virus p15 protein.

Jian He, Mingzhan Luo, Mengyang Zhang, Xiaomin Hu, Yangkun Liu, Lunguang Yao

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Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Jian He *Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Field Observation and Research Station of Headwork Wetland Ecosystem of The Central Route of South-to-North Water Diversion Project, College of Life Science, Nanyang Normal University, Nanyang, 473061, China.

Mingzhan Luo *Henan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Field Observation and Research Station of Headwork Wetland Ecosystem of The Central Route of South-to-North Water Diversion Project, College of Life Science, Nanyang Normal University, Nanyang, 473061, China.

Mengyang ZhangHenan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Field Observation and Research Station of Headwork Wetland Ecosystem of The Central Route of South-to-North Water Diversion Project, College of Life Science, Nanyang Normal University, Nanyang, 473061, China.

Xiaomin HuHenan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Field Observation and Research Station of Headwork Wetland Ecosystem of The Central Route of South-to-North Water Diversion Project, College of Life Science, Nanyang Normal University, Nanyang, 473061, China.

Yangkun LiuHenan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Field Observation and Research Station of Headwork Wetland Ecosystem of The Central Route of South-to-North Water Diversion Project, College of Life Science, Nanyang Normal University, Nanyang, 473061, China.

Lunguang YaoHenan Provincial Engineering and Technology Center of Health Products for Livestock and Poultry, Henan Field Observation and Research Station of Headwork Wetland Ecosystem of The Central Route of South-to-North Water Diversion Project, College of Life Science, Nanyang Normal University, Nanyang, 473061, China. lunguangyao@163.com.

Funding

the Science and Technology Innovation Leading Talent Project of the Central Plains of China 244200510040

6 · The paper itself

Abstract

backgroundAfrican swine fever (ASF) is a highly lethal infectious disease caused by the African swine fever virus (ASFV). Due to the absence of effective vaccines and antiviral drugs, the slaughter of infected and exposed pigs is a current prevention and control measure. Early diagnosis is crucial for the management of ASFV, and the p15 protein plays a critical role in the maturation of ASFV particles.

resultsIn this study, a prokaryotic expression system obtained highly pure soluble p15 protein, and four monoclonal antibodies (3 C, 4B, 5D, 8 F) were prepared. These antibodies demonstrated the specific recognition of exogenously expressed p15. Antigenic epitopes were initially mapped using six overlapping p15 truncated proteins. Results showed that the epitopes of 3 C, 5D, and 8 F were all located in P49-N65, which is highly conserved among genotypes I, II, VII, IX, X, and XX. In contrast, 4B identified an epitope located at K105-R132, which was conserved only in genotypes I, II, and XX ASFV.

conclusionsThis study identified two novel conserved epitopes, P49-N65 and K105-R132. This provided valuable insights into the antigenic epitopes of ASFV p15 protein, contributing to a better understanding of its functional properties and demonstrating potential utility for the development of ASFV diagnostic tools.

Indexed as

African Swine Fever VirusAntibodies, MonoclonalAntibodies, ViralViral ProteinsAfrican Swine FeverAmino Acid SequenceAnimalsEpitope MappingEpitopesMice, Inbred BALB CSwineAntibodies, MonoclonalAntibodies, ViralEpitopesViral ProteinsAfrican swine fever virusConserved epitopesMonoclonal antibodyp15 protein

Identifiers

PMID40770715

DOI10.1186/s12917-025-04954-y

PMCPMC12326869

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.